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Product Info Summary
| SKU: | M01476 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-hnRNP A1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01476
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-hnRNP A1 Rabbit Monoclonal Antibody catalog # M01476. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-hnRNP A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01476)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABEC-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human hnRNP A1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01476 is reactive to HNRNPA1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
59 kDa
Calculated molecular weight
38747 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01476 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
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Western blot analysis of hnRNP A1 expression in HepG2 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For HNRNPA1 (Source: Uniprot.org, NCBI)
Gene Name
HNRNPA1
Full Name
Heterogeneous nuclear ribonucleoprotein A1
Weight
38747 MW
Alternative Names
Heterogeneous nuclear ribonucleoprotein A1;hnRNP A1;Helix-destabilizing protein;Single-strand RNA-binding protein;hnRNP core protein A1;Heterogeneous nuclear ribonucleoprotein A1, N-terminally processed;HNRNPA1;HNRPA1; HNRNPA1 ALS19, ALS20, HNRPA1, HNRPA1L3, IBMPFD3, UP 1, hnRNP A1, hnRNP-A1 heterogeneous nuclear ribonucleoprotein A1 heterogeneous nuclear ribonucleoprotein A1|epididymis secretory sperm binding protein|helix-destabilizing protein|heterogeneous nuclear ribonucleoprotein A1B protein|heterogeneous nuclear ribonucleoprotein B2 protein|heterogeneous nuclear ribonucleoprotein core protein A1|hnRNP core protein A1-like 3|nuclear ribonucleoprotein particle A1 protein|putative heterogeneous nuclear ribonucleoprotein A1-like 3|single-strand DNA-binding protein UP1|single-strand RNA-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HNRNPA1, check out the HNRNPA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HNRNPA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-hnRNP A1 Rabbit Monoclonal Antibody (M01476)
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Customer Q&As
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5 Customer Q&As for Anti-hnRNP A1 Rabbit Monoclonal Antibody
Question
For M01476 Anti-HnRNP A1 Rabbit Monoclonal Antibody, what's the sequence of the immunogen peptide?
Verified customer
Asked: 2022-06-15
Answer
Thank you for reaching out to us. Please see the following immunogen sequence. DFGNYNNQSSNFGP
Boster Scientific Support
Answered: 2022-06-16
Question
Can you help my question with product M01476, anti-hnRNP A1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01476 anti-hnRNP A1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-29
Question
Would M01476 anti-hnRNP A1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-12-20
Answer
As indicated on the product datasheet, M01476 anti-hnRNP A1 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-20
Question
Is a blocking peptide available for product anti-hnRNP A1 Rabbit Monoclonal antibody (M01476)?
Verified Customer
Verified customer
Asked: 2018-03-16
Answer
We do provide the blocking peptide for product anti-hnRNP A1 Rabbit Monoclonal antibody (M01476). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-03-16
Question
I was wanting to use your anti-hnRNP A1 Rabbit Monoclonal antibody for WB for rat cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cervix carcinoma identification?
C. Patel
Verified customer
Asked: 2015-02-10
Answer
It shows on the product datasheet, M01476 anti-hnRNP A1 Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-02-10
