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- Table of Contents
Facts about Heterogeneous nuclear ribonucleoprotein A1.
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Human | |
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Gene Name: | HNRNPA1 |
Uniprot: | P09651 |
Entrez: | 3178 |
Belongs to: |
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No superfamily |
Helix-destabilizing protein; heterogeneous nuclear ribonucleoprotein A1; heterogeneous nuclear ribonucleoprotein A1B protein; heterogeneous nuclear ribonucleoprotein B2 protein; heterogeneous nuclear ribonucleoprotein core protein A1; hnRNP A1; hnRNP core protein A1; hnRNPA1; hnRNP-A1; HNRPA1MGC102835; nuclear ribonucleoprotein particle A1 protein; single-strand DNA-binding protein UP1; Single-strand RNA-binding protein
Mass (kDA):
38.747 kDA
Human | |
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Location: | 12q13.13 |
Sequence: | 12; NC_000012.12 (54280726..54287087) |
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes (PubMed:17289661).; Cytoplasm. (Microbial infection) In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.
The HNRNPA1 protein marker can be found in a variety of animal cell. It is a constituent of pre-mRNA and is incorporated into hnRNP particles. It may play a role the transport of poly(A mRNA from the nucleus into cytoplasm. HNRNPA1 could also bind to miRNA-hairpins which could explain its role in the transportation of mRNA.
If you're searching for high-affinity prim antibodies to detect HNRNPA1 It is recommended to look at Boster Bio products. Their primary antibodies are highly cited and have been validated in ELISA, Immunohistochemistry, and Western Blotting methods. They have a stellar track record. Find out more about Boster Bio's HNRNPA1 marker as well as other primary antibodies that are high-affinity.
Primary antibodies are immunoglobulins that recognize a specific antigen. They are made from the immune systems of hosts and are characterized by their affinity and specificity. The strength of this non-covalent bond is a measure of their affinity. Likewise, their specificity is measured by the percentage of unintended antigens they bind to. The best primary antibodies not only detect the antigen that they are interested in but also quantify, purify the antigen, quantify it, or quantify it.
HnRNPs play an important role in the regulation and the replication of viral infections. PRRSV N protein, which is essential for replication of the virus, was identified as an N protein interacting candidate that has been found to bind HnRNP F. This interaction allows HnRNP F to boost PRRSV replication and could be involved in the regulation of its replication.
Competitive EMSA can determine the binding affinity of an antibody to DNA oligos. By using unlabeled-G or T oligos, the protein-biotin-G-oligo complex shows the presence of HNF4A. ChIP tests can reveal enriched DNA sequences in fragmented chromatin. This makes the HNRNPA1 antibody superior to an IgG.
The Boster Bio Anti-HNRNPA1 antibody is a part of the Picoband(tm) catalog. This antibody is able to react with Rats, Humans and Mice. It is composed of amino acid sequences ranging from 8-42. It is also suitable for IHC–P. For optimal detection it is recommended to use multiple exposures. The following are the steps to follow when using the Boster Bio HNRNPA1 marker Western blot antibody.
First prepare first a sample. The antigen should be present in the sample. Then prepare a sample to use for Western Blotting. For Western blots, you could make use of either a monoclonal or polyclonal antibody. Monoclonal antibodies are able to recognize a single epitope of antigenicity and are more specific and have lower background than polyclonal antibodies. However, you must make sure you use the correct methods and follow the protocols precisely. Utilizing the right reagents are essential for getting precise data.
The Boster Bio HNRNPA1 marker is expressed at an equal amount in all cells and tissues. Boster's detection methods are ideal for use in Western blot. RIPA Lysis Buffer and Stripping Buffer are the reagents used in Western blot. Boster RIPA buffer can be used in many methods for purification of proteins and immunoassays. It helps prevent the interference and degrading of immunoreactivity of proteins.
The primary antibody recognizes the epitope on the target protein and then is followed by a wash step. The secondary antibody recognizes the primary antibody and is conjugated with an enzyme in order to aid the detection process. This method gives precise information about the concentrations of the targeted protein in tissues or cells. To achieve this, the secondary antibody and its derivatives are the most appropriate choice. These are both effective and cost-effective.
The hnRNPA1 gene encodes a protein which regulates the expression of virus-related genes. It is the most common hnRNP and comprises three domains. It is implicated in numerous aspects of cell RNA metabolism. It is often used in the expression of viral genes. The deregulation of the hnRNPA1 gene is associated with neurodegenerative diseases.
The Boster Bio chemistry kit used for detecting the presence of the HNRNPA1 marker allows the analysis of cellular transcripts. The method requires chromatographic separation of nuclear/cytoplasmic DNA using 1-1.2 percent agarose, electrophoresis with 1x MOPS containing 1.1 percent formaldehyde and membrane equilibration.
Boster Bio's DAB-chromic detection method by Boster Bio is a quick and simple method to determine the levels of protein in various samples. The color of the precipitate of protein is determined by measuring the amount in the samples. The system employs the enhanced HRP-DAB Chromogenic Substrate kit. It is a DMEM-GlutaMAX medium that contains the DAB substrate.
PMID: 2760922 by Biamonti G., et al. Isolation of an active gene encoding human hnRNP protein A1. Evidence for alternative splicing.
PMID: 2836799 by Buvoli M., et al. cDNA cloning of human hnRNP protein A1 reveals the existence of multiple mRNA isoforms.