HGF Human, Hepatocyte Growth Factor Human Recombinant Protein, HEK

Product Name

HGF Human, Hepatocyte Growth Factor Human Recombinant Protein, HEK

View all HGF recombinant proteins

SKU/Catalog Number

PROTP14210

Size

2ug, 10ug, 1mg

Description

HGF Human Recombinant produced in HEK cells is a glycosylated disulfide-linked heterodimer, containing 697 a.a. (Gln-32 to Ser-728) having a total molecular weight of 80kDa. The HGF is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized HGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Cite This Product

HGF Human, Hepatocyte Growth Factor Human Recombinant Protein, HEK (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP14210)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The HGF was lyophilized from a solution (1mg/ml) containing 10mM Sodium phosphate, 150mM NaCl, 0.01% Tween 80 and 100mM L-Arginine, pH 6.5.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Predicted MW

83.134kDa

Reconstitution

It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The activity was measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells (ATCC CCL-208). The EC₅₀ for this effect is typically 20-40ng/ml.

Reconstitution

It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For HGF (Source: Uniprot.org, NCBI)

Alternative Names

Scatter Factor (SF); Hepatopoietin (HPTA); HGF; HGFB; F-TCF HGF DFNB39, F-TCFB, HPTA, SF, HGF hepatocyte growth factor hepatocyte growth factor|fibroblast-derived tumor cytotoxic factor|hepatocyte growth factor (hepapoietin A; scatter factor)|hepatopoietin-A|lung fibroblast-derived mitogen

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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