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Product Info Summary
| SKU: | PB9085 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband®
View all HO-1/HMOX1/HSP32 Antibodies
SKU/Catalog Number
PB9085
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband® catalog # PB9085. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9085)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse HMOX1 recombinant protein (Position: E2-T261). Mouse HMOX1 shares 82% and 93% amino acid (aa) sequences identity with human and rat HMOX1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9085 is reactive to Hmox1 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
33 kDa
Calculated molecular weight
32929 MW
Background of HO-1/HMOX1/HSP32
HMOX1 (heme oxygenase (decycling) 1) is a human gene that encodes for the enzyme heme oxygenase 1. It is an essential enzyme in heme catabolism, it cleaves heme to form biliverdin. HMOX1 belongs to the heme oxygenase family. The HMOX1 gene is located on the long (q) arm of chromosome 22 at position 12.3, from base pair 34,101,636 to base pair 34,114,748. HMOX1, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. HMOX1 activity is induced by its substrate heme and by various nonheme substances.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9085 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat, By Heat
Positive Control
WB: rat PC-12 whole cell, mouse spleen tissue, mouse RAW2647 whole cell, mouse NIH/3T3 whole cell
IHC: mouse liver tissue, rat liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HMOX1 using anti-HMOX1 antibody (PB9085).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat PC-12 whole cell lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse RAW264.7 whole cell lysates,
Lane 4: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMOX1 antigen affinity purified polyclonal antibody (Catalog # PB9085) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMOX1 at approximately 33 kDa. The expected band size for HMOX1 is at 33 kDa.
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Figure 2. IHC analysis of HMOX1 using anti-HMOX1 antibody (PB9085).
HMOX1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMOX1 Antibody (PB9085) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of HMOX1 using anti-HMOX1 antibody (PB9085).
HMOX1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMOX1 Antibody (PB9085) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Hmox1 (Source: Uniprot.org, NCBI)
Gene Name
Hmox1
Full Name
Heme oxygenase 1
Weight
32929 MW
Superfamily
heme oxygenase family
Alternative Names
Heme oxygenase 1;HO-1;1.14.99.3;P32 protein;Hmox1; HMOX1 HMOX1D, HO-1, HSP32, bK286B10 heme oxygenase 1 heme oxygenase 1|heat shock protein, 32-kD|heme oxygenase (decycling) 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Hmox1, check out the Hmox1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Hmox1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband® (PB9085)
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