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Product Info Summary
| SKU: | M00568 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human, Monkey |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-HLA-DRB (MHC II) HLA-DRB1 Monoclonal Antibody
SKU/Catalog Number
M00568
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-HLA-DRB (MHC II) HLA-DRB1 Monoclonal Antibody (Catalog # M00568). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Monkey.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-HLA-DRB (MHC II) HLA-DRB1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00568)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM423
Isotype
IgG2b, kappa
Immunogen
Activated human peripheral blood mononuclear cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M00568 is reactive to HLA-DRB1 in Human, Monkey
Reconstitution
Reconstitute with distilled water.
Calculated molecular weight
29966 MW
Background of HLA-DRB1
This monoclonal antibody reacts with a 28kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00568 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Histiocytoma stained with Anti-HLA-DR Monoclonal Antibody (SPM423).
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Western Blot analysis of human Spleen tissue lysate using Anti-HLA-DR Monoclonal Antibody (SPM423).
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Immunofluorescence analysis of Raji cells labeling HLA-DR with HLA-DR Monoclonal Antibody (SPM423) conjugated with CF594 (Green). The nuclear counterstain is Reddot (Red)
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Flow Cytometric analysis of human Raji cells using HLA-DR MAb (SPM423) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Protein Target Info & Infographic
Gene/Protein Information For HLA-DRB1 (Source: Uniprot.org, NCBI)
Gene Name
HLA-DRB1
Full Name
HLA class II histocompatibility antigen, DRB1 beta chain
Weight
29966 MW
Alternative Names
HLA class II histocompatibility antigen, DRB1-15 beta chain;DW2.2/DR2.2;MHC class II antigen DRB1*15;HLA-DRB1;HLA-DRB2; HLA-DRB1 DRB1, HLA-DR1B, HLA-DRB, SS1 major histocompatibility complex, class II, DR beta 1 major histocompatibility complex, class II, DR beta 1 precursor|HLA class II histocompatibility , DR-1 beta chain|MHC class II HLA-DR beta 1 chain|human leucocyte DRB1|lymphocyte DRB1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HLA-DRB1, check out the HLA-DRB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HLA-DRB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HLA-DRB (MHC II) HLA-DRB1 Monoclonal Antibody (M00568)
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