Anti-HLA-DP/-DQ/-DR (MHC II) HLA-DPB1 Monoclonal Antibody

Product Name

Anti-HLA-DP/-DQ/-DR (MHC II) HLA-DPB1 Monoclonal Antibody

View all HLA-DP; HLA-DQ; HLA-DR Antibodies

SKU/Catalog Number

M30948

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-HLA-DP/-DQ/-DR (MHC II) HLA-DPB1 Monoclonal Antibody (Catalog # M30948). Tested in ELISA, Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-HLA-DP/-DQ/-DR (MHC II) HLA-DPB1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30948)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: CR3/43

Isotype

IgG1, kappa

Immunogen

Cells from human tonsil

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M30948 is reactive to HLA-DP; HLA-DQ; HLA-DR in Human

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

29159 MW

Background of HLA-DP; HLA-DQ; HLA-DR

Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36kDa) and a chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M30948 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA (For coating use Ab at 2-4ug/ml, order Ab without BSA)
Flow Cytometry (1-2ug/ million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For HLA-DP; HLA-DQ; HLA-DR (Source: Uniprot.org, NCBI)

Alternative Names

HLA class II histocompatibility antigen, DP beta 1 chain;HLA class II histocompatibility antigen, DP (W4) beta chain;MHC class II antigen DPB1;HLA-DPB1;HLA-DP1B;

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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