Click image to see more details
-
-
-
-
-
+2
Product Info Summary
| SKU: | A00194-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-HLA-B Antibody (N-term)
SKU/Catalog Number
A00194-1
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-HLA-B Antibody (N-term) (Catalog # A00194-1). Tested in IHC-P, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HLA-B Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00194-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This HLA-B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 75-107 amino acids from the N-terminal region of human HLA-B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00194-1 is reactive to HLA-B in Human
Reconstitution
Calculated molecular weight
41293 Da
Background of HLA-B
Involved in the presentation of foreign antigens to the immune system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00194-1 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
Click image to see more details
All lanes : Anti-HLA-B Antibody (N-term) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HL-60 whole cell lysate
Lane 3: A549 whole cell lysate
Lane 4: Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 40 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
All lanes : Anti-HLA-B Antibody (N-term) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HL-60 whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: Ramos whole cell lysate
Lane 5: human spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 40 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded H. tonsil section using HLA-B Antibody (N-term). A00194-1 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded H. spleen section using HLA-B Antibody (N-term). A00194-1 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded R. spleen section using HLA-B Antibody (N-term). A00194-1 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Click image to see more details
A00194-1 staining HLA-B in human tonsil tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For HLA-B (Source: Uniprot.org, NCBI)
Gene Name
HLA-B
Full Name
HLA class I histocompatibility antigen, B alpha chain
Weight
41293 Da
Alternative Names
HLA class I histocompatibility antigen, B-15 alpha chain, MHC class I antigen B*15, HLA-B, HLAB HLA-B AS, B-4901, HLAB major histocompatibility complex, class I, B major histocompatibility complex, class I, B|HLA class I HLA-B|HLA class I histocompatibility , B alpha chain|MHC HLA-B cell surface glycoprotein|MHC HLA-B transmembrane glycoprotein|MHC class 1 |MHC class I HLA-B alpha chain|MHC class I HLA-B heavy chain|MHC class I SHCHA|MHC class I molecule|leukocyte class I-B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HLA-B, check out the HLA-B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HLA-B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HLA-B Antibody (N-term) (A00194-1)
Hello CJ!
No publications found for A00194-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-HLA-B Antibody (N-term)?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-HLA-B Antibody (N-term)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
