Anti-HLA-Aw32 & HLA-A25 (MHC I) Monoclonal Antibody

Product Name

Anti-HLA-Aw32 & HLA-A25 (MHC I) Monoclonal Antibody

View all HLA-A Antibodies

SKU/Catalog Number

M00194

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-HLA-Aw32 & HLA-A25 (MHC I) Monoclonal Antibody (Catalog # M00194). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-HLA-Aw32 & HLA-A25 (MHC I) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00194)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM417

Isotype

IgG2a, kappa

Immunogen

Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, DRw10

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00194 is reactive to HLA-A in Human

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Calculated molecular weight

40460 MW

Background of HLA-A

This monoclonal antibody reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31; B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This monoclonal antibody is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00194 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For HLA-A (Source: Uniprot.org, NCBI)

Alternative Names

HLA class I histocompatibility antigen, B-7 alpha chain;MHC class I antigen B*7;HLA-B;HLAB; HLA-A HLAA major histocompatibility complex, class I, A HLA class I histocompatibility , A alpha chain|HLA class I histocompatibility , A-1 alpha chain|MHC class I HLA-A heavy chain|leukocyte class I-A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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