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Product Info Summary
| SKU: | A00194 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MHC class I HLA-A Antibody
SKU/Catalog Number
A00194
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MHC class I HLA-A Antibody catalog # A00194. Tested in WB,ICC/IF,IHC,Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MHC class I HLA-A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00194)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
recombinant protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00194 is reactive to HLA-A in Human
Reconstitution
Calculated molecular weight
40846 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00194 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:5,000
ICC: 1:100-1:500
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
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ICC staining MHC class I in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Western blot analysis of MHC class I on different lysates using anti-MHC class I antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: THP-1
Protein Target Info & Infographic
Gene/Protein Information For HLA-A (Source: Uniprot.org, NCBI)
Gene Name
HLA-A
Full Name
HLA class I histocompatibility antigen, A alpha chain
Weight
40846 MW
Superfamily
MHC class I family
Alternative Names
HLA class I histocompatibility antigen, A-1 alpha chain;MHC class I antigen A*1;HLA-A;HLAA; HLA-A HLAA major histocompatibility complex, class I, A HLA class I histocompatibility , A alpha chain|HLA class I histocompatibility , A-1 alpha chain|MHC class I HLA-A heavy chain|leukocyte class I-A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HLA-A, check out the HLA-A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HLA-A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MHC class I HLA-A Antibody (A00194)
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No publications found for A00194
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