Anti-MHC class I HLA-A Antibody

Product Name

Anti-MHC class I HLA-A Antibody

View all HLA-A Antibodies

SKU/Catalog Number

A00194

Size

100ul

Form

Liquid

Description

Boster Bio Anti-MHC class I HLA-A Antibody catalog # A00194. Tested in WB,ICC/IF,IHC,Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MHC class I HLA-A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00194)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Flt-3 around the phosphorylation site of Y969.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00194 is reactive to HLA-A in Human

Applications

A00194 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

40846 MW

Background of HLA-A

FLT3 encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

Sekine S et,al. (2008) J Immunol. ;180(12):8126-34
Pratz K et,al. (2008) Leuk Lymphoma. 2008 ;49(5):852-63.
Al Shaer L et,al. (2008) Br J Haematol. 141(4):483-93.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1,000-1:5,000
ICC: 1:100-1:500
IHC: 1:50-1:200
FC: 1:50-1:100

Validation Images & Assay Conditions

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ICC staining MHC class I in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

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Western blot analysis of MHC class I on different lysates using anti-MHC class I antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: THP-1

Gene/Protein Information For HLA-A (Source: Uniprot.org, NCBI)

Alternative Names

A-10 alpha chain; FLJ26655; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-28 alpha chain; HLA class I histocompatibility antigen, A-9 alpha chain; HLAA; major histocompatibility complex, class I, A; MHC class I antigen A*1; MHC class I antigen A*11; MHC class I antigen A*80 HLA-A HLAA major histocompatibility complex, class I, A HLA class I histocompatibility antigen, A alpha chain|HLA class I histocompatibility antigen, A-1 alpha chain|MHC class I antigen HLA-A heavy chain|leukocyte antigen class I-A

For more info on HLA-A, check out the HLA-A Infographic

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In this infographic, you will see the following information for HLA-A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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