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Product Info Summary
| SKU: | A00106-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-HLA-DQB1 Antibody Picoband®
SKU/Catalog Number
A00106-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HLA-DQB1 Antibody Picoband® catalog # A00106-1. Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HLA-DQB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00106-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human HLA-DQB1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00106-1 is reactive to HLA-DQB1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30 kDa
Calculated molecular weight
30748 MW
Background of HLA-DQB1
Major histocompatibility complex, class II, DQ beta 1, also known as HLA-DQB1, is a human gene and also denotes the genetic locus that contains this gene. HLA-DQB1 belongs to the HLA class II beta chain paralogs. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains six exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00106-1 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Positive Control
WB: human SK-OV-3 whole cell
ICC/IF: MCF-7 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HLA-DQB1 using anti-HLA-DQB1 antibody (A00106-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SK-OV-3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-DQB1 antigen affinity purified polyclonal antibody (Catalog # A00106-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA-DQB1 at approximately 30KD. The expected band size for HLA-DQB1 is at 30KD.
Click image to see more details
Figure 2. IF analysis of HLA-DQB1 using anti-HLA-DQB1 antibody (A00106-1).
HLA-DQB1 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-HLA-DQB1 Antibody (A00106-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For HLA-DQB1 (Source: Uniprot.org, NCBI)
Gene Name
HLA-DQB1
Full Name
HLA class II histocompatibility antigen, DQ beta 1 chain
Weight
30748 MW
Superfamily
MHC class II family
Alternative Names
HLA class II histocompatibility antigen, DQ beta 1 chain; MHC class II antigen DQB1; HLA-DQB1; HLA-DQB HLA-DQB1 CELIAC1, HLA-DQB, IDDM1 major histocompatibility complex, class II, DQ beta 1 HLA class II histocompatibility , DQ beta 1 chain|MHC class II DQ beta chain|MHC class II HLA-DQ beta glycoprotein|MHC class II DQB1|MHC class II HLA-DQ-beta-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HLA-DQB1, check out the HLA-DQB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HLA-DQB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HLA-DQB1 Antibody Picoband® (A00106-1)
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Customer Q&As
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5 Customer Q&As for Anti-HLA-DQB1 Antibody Picoband®
Question
Do you have a BSA free version of anti-HLA-DQB1 antibody A00106-1 available?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-HLA-DQB1 antibody A00106-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-06
Question
We are currently using anti-HLA-DQB1 antibody A00106-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
A. Krishna
Verified customer
Asked: 2017-10-31
Answer
The anti-HLA-DQB1 antibody (A00106-1) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-31
Question
My lab would like to test anti-HLA-DQB1 antibody A00106-1 on human peripheral blood for research purposes, then I may be interested in using anti-HLA-DQB1 antibody A00106-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Johnson
Verified customer
Asked: 2016-08-31
Answer
The products we sell, including anti-HLA-DQB1 antibody A00106-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-08-31
Question
Will anti-HLA-DQB1 antibody A00106-1 work on primate WB with b-cell?
C. Wu
Verified customer
Asked: 2016-07-19
Answer
Our lab technicians have not validated anti-HLA-DQB1 antibody A00106-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-HLA-DQB1 antibody A00106-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate b-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-07-19
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for peripheral blood using anti-HLA-DQB1 antibody A00106-1. Let me know if you need anything else.
J. Carter
Verified customer
Asked: 2014-01-27
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-01-27
