Anti-HINT1 Antibody

Product Name

Anti-HINT1 Antibody

View all HINT1 Antibodies

SKU/Catalog Number

M02557

Size

80 µl

Description

Boster Bio Anti-HINT1 Antibody (Catalog # M02557). Tested in WB, Flow Cytometry, IHC-P, IF application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-HINT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02557)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1500CT836.13.93

Isotype

IgG1,k

Immunogen

This HINT1 antibody is generated from a mouse immunized with a recombinant protein of human HINT1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02557 is reactive to HINT1 in Human, Mouse, Rat

Applications

M02557 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

13802 Da

Background of HINT1

Hydrolyzes purine nucleotide phosphoramidates with a single phosphate group, including adenosine 5'monophosphoramidate (AMP-NH2), adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His- AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O- phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin- protein ligase complex.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:25
WB: 1:4000
IHC-P: 1:25
FC: 1:25

Validation Images & Assay Conditions

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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma cell line) cells labeling HINT1 with M02557 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

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All lanes : Anti-HINT1 Antibody at 1:4000 dilution
Lane 1: Jurkat whole cell lysates
Lane 2: Raji whole cell lysates
Lane 3: HepG2 whole cell lysates
Lane 4: rat brain lysates
Lane 5: mouse liver lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 14 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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M02557 staining HINT1 in human kidney sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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Overlay histogram showing Jurkat cells stained with M02557 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M02557, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Gene/Protein Information For HINT1 (Source: Uniprot.org, NCBI)

Alternative Names

Adenosine 5'-monophosphoramidase; EC 3.-; HINT; histidine triad nucleotide binding protein 1; histidine triad nucleotide-binding protein 1; histidine triad nucleotide-binding protein; PKCI1; PKCI-1FLJ30414; PRKCNH1FLJ32340; Protein kinase C inhibitor 1; Protein kinase C-interacting protein 1 HINT1 HINT, NMAN, PKCI-1, PRKCNH1 histidine triad nucleotide binding protein 1 histidine triad nucleotide-binding protein 1|adenosine 5-monophosphoramidase|epididymis secretory sperm binding protein|protein kinase C inhibitor 1|protein kinase C-interacting protein 1

For more info on HINT1, check out the HINT1 Infographic

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In this infographic, you will see the following information for HINT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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