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Product Info Summary
| SKU: | A01813-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Kv1.1 potassium channel/KCNA1 Antibody Picoband®
SKU/Catalog Number
A01813-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Kv1.1 potassium channel/KCNA1 Antibody Picoband® catalog # A01813-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Kv1.1 potassium channel/KCNA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01813-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Kv1.1 potassium channel/KCNA1 recombinant protein (Position: M1-V495).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01813-3 is reactive to KCNA1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
90 kDa
Calculated molecular weight
56.466kDa
Background of Kv1.1
Potassium voltage-gated channel subfamily A member 1, also known as Kv1.1, is a shaker related voltage-gated potassium channel that in humans is encoded by the KCNA1 gene. It is mapped to 12p13.32. The protein functions as a potassium selective channel through which the potassium ion may pass through in consensus with the electrochemical gradient. The N-terminus of the channel is associated with beta subunits that can modify the inactivation properties of the channel as well as affect expression levels. The C-terminus of the channel is complexed to a PDZ domain protein that is responsible for channel targeting.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01813-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
FCM: U20S cell, U87 cell
Validation Images & Assay Conditions
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Figure 2. Flow Cytometry analysis of U20S cells using anti-KCNA1 antibody (A01813-3).
Overlay histogram showing U20S cells stained with A01813-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KCNA1 Antibody (A01813-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 3. Flow Cytometry analysis of U87 cells using anti-KCNA1 antibody (A01813-3).
Overlay histogram showing U87 cells stained with A01813-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-KCNA1 Antibody (A01813-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For KCNA1 (Source: Uniprot.org, NCBI)
Gene Name
KCNA1
Full Name
Potassium voltage-gated channel subfamily A member 1
Weight
56.466kDa
Superfamily
potassium channel family
Alternative Names
Potassium voltage-gated channel subfamily A member 1; Voltage-gated K (+) channel HuKI KCNA1 AEMK, EA1, HBK1, HUK1, KV1.1, MBK1, MK1, RBK1 potassium voltage-gated channel subfamily A member 1 potassium voltage-gated channel subfamily A member 1|potassium channel, voltage gated shaker related subfamily A, member 1|potassium voltage-gated channel, shaker-related subfamily, member 1 (episodic ataxia with myokymia)|voltage-gated K(+) channel HuKI|voltage-gated potassium channel HBK1|voltage-gated potassium channel subunit Kv1.1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KCNA1, check out the KCNA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KCNA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Kv1.1 potassium channel/KCNA1 Antibody Picoband® (A01813-3)
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