Anti-HINT1 Antibody

Product Name

Anti-HINT1 Antibody

View all HINT1 Antibodies

SKU/Catalog Number

A02557

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-HINT1 Antibody (Catalog # A02557). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

HINT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-HINT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02557)

Host

Rabbit

Contents

HINT1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

HINT1 antibody was raised against a 17 amino acid synthetic peptide near the center of the human HINT1. The immunogen is located within the last 50 amino acids of HINT1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms of TNFSF4 are known to exist; this antibody will detect both isoforms.

Reactive Species

A02557 is reactive to HINT1 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

13802 MW

Background of HINT1

Histidine triad nucleotide-binding protein 1 (HINT1) is a member of the histidine triad (HIT) protein family, a group of small nucleotide-binding and -hydrolyzing proteins. HINT1 interacts with several diverse proteins and has been suggested to have tumor suppressive activities. HINT1 catalyzes the hydrolysis of adenosine 5'-monophoramidate substrates such as AMP-morpholidate, but its enzymatic function does not appear to play a role in its tumor suppression. Recent experiments demonstrate that HINT1 forms a complex with POSH and JNK in vivo, inhibiting AP-1 activity and the phosphorylation of c-Jun, and this action could contribute to the tumor suppressor activity of HINT1. Other studies raise the possibility of HINT1 as a candidate gene for schizophrenia.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02557 is guaranteed for ELISA, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

HINT1 antibody can be used for detection of HINT1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For HINT1 (Source: Uniprot.org, NCBI)

Alternative Names

HINT, NMAN, PKCI-1, PRKCNH1, HINT, PKCI1, Histidine triad nucleotide-binding protein 1, Protein kinase C inhibitor 1 HINT1 HINT, NMAN, PKCI-1, PRKCNH1 histidine triad nucleotide binding protein 1 histidine triad nucleotide-binding protein 1|adenosine 5-monophosphoramidase|epididymis secretory sperm binding protein|protein kinase C inhibitor 1|protein kinase C-interacting protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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