Anti-SERCA1 ATPase/ATP2A1 Antibody Picoband®

Product Name

Anti-SERCA1 ATPase/ATP2A1 Antibody Picoband®

View all SERCA1 ATPase Antibodies

SKU/Catalog Number

PA1719

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SERCA1 ATPase/ATP2A1 Antibody catalog # PA1719. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SERCA1 ATPase/ATP2A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1719)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human SERCA1 ATPase, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1719 is reactive to ATP2A1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

29 kDa

Calculated molecular weight

110252 MW

Background of SERCA1 ATPase

SERCA1 (SARCOPLASMIC RETICULUM Ca (2+)-ATPase 1), also called ATP2A1, is an enzyme that in humans is encoded by the ATP2A1 gene. This gene encodes one of the SERCA Ca (2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. The SERCA1 gene is mapped on 16p11.2. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Zhang et al. (1995)determined that the human ATP2A1 gene is 26 kb long and contains 23 exons, 1 of which can be alternatively spliced.Overexpression of S1T, but not full-length SERCA1, induced ER stress in HeLa cells and amplified ER stress through the PERK (EIF2AK3)-EIF2A -ATF4 -CHOP (DDIT3) pathway. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1719 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: Rat Skeletal Muscle Tissue, PANC Cell, U87 Cell,
IHC: Human Placenta Tissue, Rat Skeletal Muscle Tissue

Validation Images & Assay Conditions

Gene/Protein Information For ATP2A1 (Source: Uniprot.org, NCBI)

Alternative Names

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1;SERCA1;SR Ca (2+)-ATPase 1;3.6.3.8;Calcium pump 1;Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform;Endoplasmic reticulum class 1/2 Ca (2+) ATPase;ATP2A1; ATP2A1 ATP2A, SERCA1 ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 sarcoplasmic/endoplasmic reticulum calcium ATPase 1|ATPase, Ca++ transporting, cardiac muscle, fast twitch 1|SR Ca(2+)-ATPase 1|calcium pump 1|endoplasmic reticulum class 1/2 Ca(2+) ATPase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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