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Product Info Summary
| SKU: | A00539-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Her3/ErbB3 (Ab-1328) Antibody
View all ErbB3/Her3 Antibodies
SKU/Catalog Number
A00539-2
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Her3/ErbB3 (Ab-1328) Antibody (Catalog # A00539-2). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Her3/ErbB3 (Ab-1328) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00539-2)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa. 1326~1330 (P-D-Y-W-H) derived from Human Her3/ErbB3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00539-2 is reactive to ERBB3 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
51778 MW
Background of ErbB3/Her3
Binds and is activated by neuregulins and NTAK.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00539-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 185kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ERBB3 using anti-ERBB3 antibody (A00539-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERBB3 antigen affinity purified polyclonal antibody (Catalog # A00539-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ERBB3.
Click image to see more details
Figure 2. IHC analysis of ERBB3 using anti-ERBB3 antibody (A00539-2).
ERBB3 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ERBB3 Antibody (A00539-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ERBB3 (Source: Uniprot.org, NCBI)
Gene Name
ERBB3
Full Name
Receptor tyrosine-protein kinase erbB-3
Weight
51778 MW
Superfamily
protein kinase superfamily
Alternative Names
Coagulation factor IX ;3.4.21.22 ;Christmas factor;Plasma thromboplastin component;PTC;Coagulation factor IXa light chain;Coagulation factor IXa heavy chain;F9; ERBB3 ErbB-3, FERLK, HER3, LCCS2, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3 erb-b2 receptor tyrosine kinase 3 receptor tyrosine-protein kinase erbB-3|human epidermal growth factor receptor 3|proto-oncogene-like protein c-ErbB-3|tyrosine kinase-type cell surface receptor HER3|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERBB3, check out the ERBB3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERBB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Her3/ErbB3 (Ab-1328) Antibody (A00539-2)
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No publications found for A00539-2
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Her3/ErbB3 (Ab-1328) Antibody
Question
Will A00539-2 anti-Her3/ErbB3 (Ab-1328) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Z. Jackson
Verified customer
Asked: 2020-03-27
Answer
It shows on the product datasheet, A00539-2 anti-Her3/ErbB3 (Ab-1328) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-27
Question
Would anti-Her3/ErbB3 (Ab-1328) antibody A00539-2 work for WB with liver?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
According to the expression profile of liver, ERBB3 is highly expressed in liver. So, it is likely that anti-Her3/ErbB3 (Ab-1328) antibody A00539-2 will work for WB with liver.
Boster Scientific Support
Answered: 2020-03-23
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Her3/ErbB3 (Ab-1328) antibody A00539-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-19
Question
We have been able to see staining in rat placenta small intestine. Any tips? Is anti-Her3/ErbB3 (Ab-1328) antibody supposed to stain placenta small intestine positively?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
According to literature placenta small intestine does express ERBB3. According to Uniprot.org, ERBB3 is expressed in corpus callosum, placenta, hippocampus, placenta small intestine, skin, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have ERBB3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Placenta, and Small intestine, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7685162
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-05
Question
Our lab used your anti-Her3/ErbB3 (Ab-1328) antibody for WB on skin in the past. I am using mouse, and I plan to use the antibody for IHC next. I am looking for examining skin as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
M. Singh
Verified customer
Asked: 2020-02-14
Answer
I took a look at the website and datasheets of our anti-Her3/ErbB3 (Ab-1328) antibody and it seems that A00539-2 has been validated on mouse in both WB and IHC. Thus A00539-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-14
Question
See below the WB image, lot number and protocol we used for liver using anti-Her3/ErbB3 (Ab-1328) antibody A00539-2. Please let me know if you require anything else.
D. Krishna
Verified customer
Asked: 2019-11-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-21
Question
I see that the anti-Her3/ErbB3 (Ab-1328) antibody A00539-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-11
Question
Do you have a BSA free version of anti-Her3/ErbB3 (Ab-1328) antibody A00539-2 available?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Her3/ErbB3 (Ab-1328) antibody A00539-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-26
Question
We are currently using anti-Her3/ErbB3 (Ab-1328) antibody A00539-2 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
The anti-Her3/ErbB3 (Ab-1328) antibody (A00539-2) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-02
Question
I have a question about product A00539-2, anti-Her3/ErbB3 (Ab-1328) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-07
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00539-2 anti-Her3/ErbB3 (Ab-1328) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-07
Question
I was wanting to use your anti-Her3/ErbB3 (Ab-1328) antibody for WB for mouse liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse liver identification?
J. Collins
Verified customer
Asked: 2018-11-01
Answer
As indicated on the product datasheet, A00539-2 anti-Her3/ErbB3 (Ab-1328) antibody has been tested for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-01
Question
Is a blocking peptide available for product anti-Her3/ErbB3 (Ab-1328) antibody (A00539-2)?
Verified Customer
Verified customer
Asked: 2018-08-03
Answer
We do provide the blocking peptide for product anti-Her3/ErbB3 (Ab-1328) antibody (A00539-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-08-03
Question
We want to test anti-Her3/ErbB3 (Ab-1328) antibody A00539-2 on mouse liver for research purposes, then I may be interested in using anti-Her3/ErbB3 (Ab-1328) antibody A00539-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
The products we sell, including anti-Her3/ErbB3 (Ab-1328) antibody A00539-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-29
Question
Is this A00539-2 anti-Her3/ErbB3 (Ab-1328) antibody reactive to the isotypes of ERBB3?
A. Edwards
Verified customer
Asked: 2014-10-24
Answer
The immunogen of A00539-2 anti-Her3/ErbB3 (Ab-1328) antibody is Peptide sequence around aa. 1326~1330 (P-D-Y-W-H) derived from Human Her3/ErbB3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-10-24
Question
My colleagues were well pleased with the WB result of your anti-Her3/ErbB3 (Ab-1328) antibody. However we have been able to see positive staining in placenta small intestine isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is ERBB3 supposed to be expressed?
D. Parker
Verified customer
Asked: 2013-10-21
Answer
Based on literature, placenta small intestine does express ERBB3. Generally ERBB3 expresses in isoform 1: cell membrane. Regarding which tissues have ERBB3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Placenta, and Small intestine, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7685162
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-10-21
