Click image to see more details
Product Info Summary
| SKU: | A00539-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF |
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Product info
Product Name
Anti-Her3/ErbB3 (Ab-1289) Antibody
View all ErbB3/Her3 Antibodies
SKU/Catalog Number
A00539-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Her3/ErbB3 (Ab-1289) Antibody (Catalog # A00539-1). Tested in IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Her3/ErbB3 (Ab-1289) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00539-1)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.1287~1291 (Q-G-Y-E-E) derived from Human Her3/ErbB3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00539-1 is reactive to ERBB3 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
51778 MW
Background of ErbB3/Her3
Binds and is activated by neuregulins and NTAK.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00539-1 is guaranteed for IF Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 185kd
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Immunofluorescence staining of methanol-fixed MCF cells using Her3/ErbB3(Ab-1289) Antibody #A00539-1.
Protein Target Info & Infographic
Gene/Protein Information For ERBB3 (Source: Uniprot.org, NCBI)
Gene Name
ERBB3
Full Name
Receptor tyrosine-protein kinase erbB-3
Weight
51778 MW
Superfamily
protein kinase superfamily
Alternative Names
Coagulation factor IX ;3.4.21.22 ;Christmas factor;Plasma thromboplastin component;PTC;Coagulation factor IXa light chain;Coagulation factor IXa heavy chain;F9; ERBB3 ErbB-3, FERLK, HER3, LCCS2, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3 erb-b2 receptor tyrosine kinase 3 receptor tyrosine-protein kinase erbB-3|human epidermal growth factor receptor 3|proto-oncogene-like protein c-ErbB-3|tyrosine kinase-type cell surface receptor HER3|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERBB3, check out the ERBB3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERBB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Her3/ErbB3 (Ab-1289) Antibody (A00539-1)
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Customer Q&As
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10 Customer Q&As for Anti-Her3/ErbB3 (Ab-1289) Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta small intestine using anti-Her3/ErbB3 (Ab-1289) antibody A00539-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-31
Question
We are currently using anti-Her3/ErbB3 (Ab-1289) antibody A00539-1 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
The anti-Her3/ErbB3 (Ab-1289) antibody (A00539-1) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-04
Question
I am interested in using your anti-Her3/ErbB3 (Ab-1289) antibody for schwann cell differentiation studies. Has this antibody been tested with western blotting on mcf cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
Thanks for your inquiry. This A00539-1 anti-Her3/ErbB3 (Ab-1289) antibody is validated on mcf cells. It is guaranteed to work for IF in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-06
Question
I am looking for to test anti-Her3/ErbB3 (Ab-1289) antibody A00539-1 on human placenta small intestine for research purposes, then I may be interested in using anti-Her3/ErbB3 (Ab-1289) antibody A00539-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-10-31
Answer
The products we sell, including anti-Her3/ErbB3 (Ab-1289) antibody A00539-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-10-31
Question
We have observed staining in human corpus callosum. Any tips? Is anti-Her3/ErbB3 (Ab-1289) antibody supposed to stain corpus callosum positively?
Verified Customer
Verified customer
Asked: 2018-09-28
Answer
From what I have seen in literature corpus callosum does express ERBB3. From what I have seen in Uniprot.org, ERBB3 is expressed in corpus callosum, placenta, hippocampus, placenta small intestine, skin, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have ERBB3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Placenta, and Small intestine, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7685162
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-09-28
Question
See below the WB image, lot number and protocol we used for placenta small intestine using anti-Her3/ErbB3 (Ab-1289) antibody A00539-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-02-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-05
Question
I was wanting to use your anti-Her3/ErbB3 (Ab-1289) antibody for IF for human placenta small intestine on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human placenta small intestine identification?
Verified Customer
Verified customer
Asked: 2017-11-17
Answer
As indicated on the product datasheet, A00539-1 anti-Her3/ErbB3 (Ab-1289) antibody has been validated for IF on human tissues. We have an innovator award program that if you test this antibody and show it works in human placenta small intestine in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-17
Question
My team were well pleased with the WB result of your anti-Her3/ErbB3 (Ab-1289) antibody. However we have been able to see positive staining in hippocampus isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is ERBB3 supposed to be expressed?
A. Anderson
Verified customer
Asked: 2017-05-10
Answer
From literature, hippocampus does express ERBB3. Generally ERBB3 expresses in isoform 1: cell membrane. Regarding which tissues have ERBB3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Placenta, and Small intestine, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7685162
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-05-10
Question
Is this A00539-1 anti-Her3/ErbB3 (Ab-1289) antibody reactive to the isotypes of ERBB3?
S. Bhatt
Verified customer
Asked: 2015-10-16
Answer
The immunogen of A00539-1 anti-Her3/ErbB3 (Ab-1289) antibody is Peptide sequence around aa.1287~1291 (Q-G-Y-E-E) derived from Human Her3/ErbB3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-10-16
Question
Would anti-Her3/ErbB3 (Ab-1289) antibody A00539-1 work for IF with placenta small intestine?
K. Miller
Verified customer
Asked: 2014-04-14
Answer
According to the expression profile of placenta small intestine, ERBB3 is highly expressed in placenta small intestine. So, it is likely that anti-Her3/ErbB3 (Ab-1289) antibody A00539-1 will work for IF with placenta small intestine.
Boster Scientific Support
Answered: 2014-04-14
