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Product Info Summary
| SKU: | PA1880 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ErbB 3/ERBB3 Antibody Picoband®
View all ErbB3/Her3 Antibodies
SKU/Catalog Number
PA1880
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ErbB 3/ERBB3 Antibody catalog # PA1880. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ErbB 3/ERBB3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1880)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ErbB 3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1880 is reactive to ERBB3 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
180 kDa
Calculated molecular weight
148098 MW
Background of ErbB3/Her3
ERBB3 (V-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3) also known as ONCOGENE ERBB3 or HER3, is an enzyme that in humans is encoded by the ERBB3 gene. This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. By in situ hybridization, Kraus et al. (1989) mapped the ERBB3 gene to chromosome 12q13. Carraway et al. (1994) demonstrated that ERBB3 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Most notably, EGFR and ErbB2 became markedly more promiscuous as the threshold was lowered, whereas ErbB3did not. Engelman et al. (2007) found that amplification of MET caused gefitinib resistance by driving ERBB3-dependent activation of phosphoinositide 3-kinase, a pathway though to be specific to EGFR/ERBB family receptors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1880 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Caco-2 whole cell, human K562 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ERBB3 using anti-ERBB3 antibody (PA1880).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates
Lane 2: human K562 whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERBB3 antigen affinity purified polyclonal antibody (Catalog # PA1880) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERBB3 at approximately 180KD. The expected band size for ERBB3 is at 148KD.
Protein Target Info & Infographic
Gene/Protein Information For ERBB3 (Source: Uniprot.org, NCBI)
Gene Name
ERBB3
Full Name
Receptor tyrosine-protein kinase erbB-3
Weight
148098 MW
Superfamily
protein kinase superfamily
Alternative Names
Receptor tyrosine-protein kinase erbB-3;2.7.10.1;Proto-oncogene-like protein c-ErbB-3;Tyrosine kinase-type cell surface receptor HER3;ERBB3;HER3; ERBB3 ErbB-3, FERLK, HER3, LCCS2, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3 erb-b2 receptor tyrosine kinase 3 receptor tyrosine-protein kinase erbB-3|human epidermal growth factor receptor 3|proto-oncogene-like protein c-ErbB-3|tyrosine kinase-type cell surface receptor HER3|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERBB3, check out the ERBB3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERBB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ErbB 3/ERBB3 Antibody Picoband® (PA1880)
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Customer Q&As
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4 Customer Q&As for Anti-ErbB 3/ERBB3 Antibody Picoband®
Question
We are currently using anti-ErbB 3/ERBB3 antibody PA1880 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
The anti-ErbB 3/ERBB3 antibody (PA1880) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-24
Question
We have been able to see staining in human placenta. Do you have any suggestions? Is anti-ErbB 3/ERBB3 antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
From literature placenta does express ERBB3. From Uniprot.org, ERBB3 is expressed in corpus callosum, placenta, hippocampus, placenta small intestine, skin, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have ERBB3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Placenta, and Small intestine, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7685162
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-13
Question
I would like using your anti-ErbB 3/ERBB3 antibody for schwann cell differentiation studies. Has this antibody been tested with western blotting on k562 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
Thanks for your inquiry. This PA1880 anti-ErbB 3/ERBB3 antibody is validated on human k562, k562 whole cell lysates. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-18
Question
We were happy with the WB result of your anti-ErbB 3/ERBB3 antibody. However we have observed positive staining in placenta small intestine isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is ERBB3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
From what I have seen in literature, placenta small intestine does express ERBB3. Generally ERBB3 expresses in isoform 1: cell membrane. Regarding which tissues have ERBB3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Placenta, and Small intestine, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7685162
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-06-25
