Anti-HEC1 NDC80 Monoclonal Antibody

Product Name

Anti-HEC1 NDC80 Monoclonal Antibody

View all NDC80 Antibodies

SKU/Catalog Number

M01731

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-HEC1 NDC80 Monoclonal Antibody catalog # M01731. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HEC1 NDC80 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01731)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ACBI-14

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human HEC1 Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01731 is reactive to NDC80 in Human, Mouse, Rat

Applications

M01731 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

16832 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IP 1:50

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of HEC1 expression in Jurkat cell lysate.

Gene/Protein Information For NDC80 (Source: Uniprot.org, NCBI)

Alternative Names

HEC1KNTC2; HECRetinoblastoma-associated protein HEC; Highly expressed in cancer protein; highly expressed in cancer, rich in leucine heptad repeats (yeast); highly expressed in cancer, rich in leucine heptad repeats; hsHec1; hsNDC80; kinetochore associated 2; Kinetochore protein Hec1; kinetochore protein NDC80 homolog; Kinetochore-associated protein 2; NDC80 homolog, kinetochore complex component (S. cerevisiae); TID3 NDC80 HEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80 NDC80 kinetochore complex component kinetochore protein NDC80 homolog|NDC80 homolog, kinetochore complex component|NDC80 kinetochore complex component homolog|highly expressed in cancer protein|highly expressed in cancer, rich in leucine heptad repeats|kinetochore associated 2|kinetochore protein Hec1|kinetochore-associated protein 2|retinoblastoma-associated protein HEC

For more info on NDC80, check out the NDC80 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NDC80: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the NDC80 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis