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Product Info Summary
| SKU: | M01731 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-HEC1 NDC80 Monoclonal Antibody
SKU/Catalog Number
M01731
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HEC1 NDC80 Monoclonal Antibody catalog # M01731. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HEC1 NDC80 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01731)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACBI-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HEC1 Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01731 is reactive to NDC80 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
68 kDa
Calculated molecular weight
16832 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01731 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IP 1:50
Positive Control
WB: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of HEC1 expression in Jurkat cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For NDC80 (Source: Uniprot.org, NCBI)
Gene Name
NDC80
Full Name
Kinetochore protein NDC80 homolog
Weight
16832 MW
Superfamily
NDC80/HEC1 family
Alternative Names
Eukaryotic translation initiation factor 5A-1;eIF-5A-1;eIF-5A1;Eukaryotic initiation factor 5A isoform 1;eIF-5A;Rev-binding factor;eIF-4D;EIF5A; NDC80 HEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80 NDC80 kinetochore complex component kinetochore protein NDC80 homolog|NDC80 homolog, kinetochore complex component|NDC80 kinetochore complex component homolog|highly expressed in cancer protein|highly expressed in cancer, rich in leucine heptad repeats|kinetochore associated 2|kinetochore protein Hec1|kinetochore-associated protein 2|retinoblastoma-associated protein HEC
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NDC80, check out the NDC80 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NDC80: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HEC1 NDC80 Monoclonal Antibody (M01731)
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No publications found for M01731
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Customer Q&As
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4 Customer Q&As for Anti-HEC1 NDC80 Monoclonal Antibody
Question
Is this M01731 anti-HEC1 Monoclonal antibody reactive to the isotypes of NDC80?
M. Johnson
Verified customer
Asked: 2019-03-25
Answer
The immunogen of M01731 anti-HEC1 Monoclonal antibody is A synthesized peptide derived from human HEC1 Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-03-25
Question
Will anti-HEC1 Monoclonal antibody M01731 work on primate IP with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2018-07-19
Answer
Our lab technicians have not tested anti-HEC1 Monoclonal antibody M01731 on primate. You can run a BLAST between primate and the immunogen sequence of anti-HEC1 Monoclonal antibody M01731 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate cervix carcinoma in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-19
Question
I was wanting to use to test anti-HEC1 Monoclonal antibody M01731 on human cervix carcinoma for research purposes, then I may be interested in using anti-HEC1 Monoclonal antibody M01731 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Jones
Verified customer
Asked: 2014-08-29
Answer
The products we sell, including anti-HEC1 Monoclonal antibody M01731, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-08-29
Question
I see that the anti-HEC1 Monoclonal antibody M01731 works with IP, what is the protocol used to produce the result images on the product page?
M. Johnson
Verified customer
Asked: 2014-03-11
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-03-11
