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Product Info Summary
| SKU: | A07023 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ZWINT Antibody Picoband®
SKU/Catalog Number
A07023
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ZWINT Antibody Picoband® catalog # A07023. Tested in ELISA, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZWINT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07023)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat ZWINT recombinant protein (Position: M1-D253).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07023 is reactive to Zwint in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
31.293kDa
Background of ZWINT
ZW10 interactor (Zwint-1) is a protein that in humans is encoded by the ZWINT gene. This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07023 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Rat
ELISA, 0.1-0.5μg/ml,
Positive Control
WB: rat thymus tissue, rat liver tissue, rat testis tissue, rat RH35 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ZWINT using anti-ZWINT antibody (A07023).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: rat testis tissue lysates,
Lane 4: rat RH35 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZWINT antigen affinity purified polyclonal antibody (Catalog # A07023) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZWINT at approximately 38KD. The expected band size for ZWINT is at 38KD.
Protein Target Info & Infographic
Gene/Protein Information For Zwint (Source: Uniprot.org, NCBI)
Gene Name
Zwint
Full Name
ZW10 interactor
Weight
31.293kDa
Alternative Names
Forkhead box protein F1; Forkhead-related activator 1; FREAC-1; Forkhead-related protein FKHL5; Forkhead-related transcription factor 1; FOXF1; FKHL5; FREAC1 ZWINT HZwint-1, KNTC2AP, SIP301, ZWINT ZW10 interacting kinetochore protein ZW10 interactor|SNAP25 interacting protein of 30 kDa|ZW10 interactor, kinetochore protein|human ZW10 interacting protein-1|zwint-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Zwint, check out the Zwint Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Zwint: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZWINT Antibody Picoband® (A07023)
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