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Product Info Summary
| SKU: | PB9607 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-KIN Antibody Picoband®
SKU/Catalog Number
PB9607
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KIN Antibody Picoband® catalog # PB9607. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KIN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9607)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human KIN recombinant protein (Position: K3-K202). Human KIN shares 98% amino acid (aa) sequence identity with mouse KIN.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9607 is reactive to KIN in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
45374 MW
Background of Kin
DNA/RNA-binding protein KIN17, also known as BTCD or KIN17, is a protein that in humans is encoded by the KIN gene. This gene is mapped to 10p14. The protein encoded by this gene is a nuclear protein that forms intranuclear foci during proliferation and is redistributed in the nucleoplasm during the cell cycle. Short-wave ultraviolet light provokes the relocalization of the protein, suggesting its participation in the cellular response to DNA damage. Originally selected based on protein-binding with RecA antibodies, the mouse protein presents a limited similarity with a functional domain of the bacterial RecA protein, a characteristic shared by this human ortholog. Alternative splicing of this gene results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9607 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human 293T whole cell, human HepG2 whole cell, rat RH35 whole cell, rat NRK whole cell, mouse HEPA1-6 whole cell, mouse NIH/3T3 whole cell
IHC: human oesophagus squama cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of KIN using anti-KIN antibody (PB9607).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: rat NRK whole cell lysates,
Lane 7: mouse HEPA1-6 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIN antigen affinity purified polyclonal antibody (Catalog # PB9607) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIN at approximately 45 kDa. The expected band size for KIN is at 45 kDa.
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Figure 2. IHC analysis of KIN using anti-KIN antibody (PB9607).
KIN was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-KIN Antibody (PB9607) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For KIN (Source: Uniprot.org, NCBI)
Gene Name
KIN
Full Name
DNA/RNA-binding protein KIN17
Weight
45374 MW
Superfamily
KIN17 family
Alternative Names
DNA/RNA-binding protein KIN17;Binding to curved DNA;KIN, antigenic determinant of recA protein homolog;KIN ;BTCD, KIN17; Kin|Kin17|Kin17 DNA and RNA binding protein|DNA/RNA-binding protein KIN17|KIN, ic determinant of recA protein homolog|ic determinant of rec-A protein|binding to curved DNA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KIN, check out the KIN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KIN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KIN Antibody Picoband® (PB9607)
Hello CJ!
PB9607 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen,J.,Xia,Y.,Peng,Y.,Wu,S.,Liu,W.,Zhang,H. ... Zhao,L.(2021).Analysis of the association between KIN17 expression and the clinical features/prognosis of epithelial ovarian cancer, and the effects of KIN17 in SKOV3 cells.Oncology Letters,21,475.https://doi.org/10.3892/ol.2021.12736
Species: Human
PB9607 usage in article: APP:WB, SAMPLE:EOC TISSUE, DILUTION:1:2000
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Customer Q&As
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3 Customer Q&As for Anti-KIN Antibody Picoband®
Question
Is this PB9607 anti-KIN antibody reactive to the isotypes of KIN?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
The immunogen of PB9607 anti-KIN antibody is E.coli-derived human KIN recombinant protein (Position: K3-K202). Human KIN shares 98% amino acid (aa) sequence identity with mouse KIN. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-16
Question
Can you help my question with product PB9607, anti-KIN antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9607 anti-KIN antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-18
Question
We are currently using anti-KIN antibody PB9607 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on canine tissues as well?
J. Wu
Verified customer
Asked: 2019-02-15
Answer
The anti-KIN antibody (PB9607) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-15
