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Product Info Summary
| SKU: | M09275 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Heat Shock Protein 27 Monoclonal Antibody
SKU/Catalog Number
M09275
Size
100ul
Description
Boster Bio Anti-Heat Shock Protein 27 Monoclonal Antibody catalog # M09275. Tested in IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Heat Shock Protein 27 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09275)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Recombinant full length human HSP27 from E. coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M09275 is reactive to HSBP1 in Human
Reconstitution
Calculated molecular weight
8544 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09275 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:10,000. IF/ICC and IHC: 1:1,000.
Validation Images & Assay Conditions
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Western blot of tissue or whole cell lysates using mouse mAb to HSP27, M09275, dilution 1:10,000, in green. [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong single band at ~27kDa corresponds to the HSP27 protein, detected only in human cell lines, since this antibody is human specific. The blot was simultaneously probed with rabbit pAb to HSP-60, dilution 1:5,000 in red. A strong 60kDa band is present in all preparations.
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Immunofluorescent analysis of HeLa cells stained with mouse mAb to HSP27, M09275, dilution 1:2,000 in green, and costained with chicken pAb to HSP60, dilution 1:2,000 in red. Blue is DAPI staining of nuclear DNA. The M09275 antibody produces strong cytoplasm staining, while the HSP60 antibody specifically labels mitochondria.
Protein Target Info & Infographic
Gene/Protein Information For HSBP1 (Source: Uniprot.org, NCBI)
Gene Name
HSBP1
Full Name
Heat shock factor-binding protein 1
Weight
8544 MW
Superfamily
HSBP1 family
Alternative Names
Heat shock factor-binding protein 1;Nasopharyngeal carcinoma-associated antigen 13;NPC-A-13;HSBP1;HSF1BP; HSBP1 NPC-A-13 heat shock factor binding protein 1 heat shock factor-binding protein 1|nasopharyngeal carcinoma-associated 13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSBP1, check out the HSBP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSBP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Heat Shock Protein 27 Monoclonal Antibody (M09275)
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