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Product Info Summary
| SKU: | EK7109 |
|---|---|
| Size: | 96 wells/kit |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Plasma, Cell Lysates, Tissue |
Product info
Product Name
HSP70 ELISA Kit (High-Sensitivity)
SKU/Catalog Number
EK7109
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
High-Sensitivity Colorimetric detection of HSP70. 96 wells/kit.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
HSP70 ELISA Kit (High-Sensitivity) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7109)
Clonality of Antibodies
See Datasheet for details
Sensitivity
0.038 ng/ml
Assay Range
0.42 - 27 ng/ml
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7109 is reactive to HSP70 in Dog, Human, Monkey, Mouse, Rat samples
Validated Sample Types
Plasma, Cell Lysates, Tissue
Application Guarantee
EK7109 is guaranteed for ELISA in Dog, Human, Monkey, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Description | Quantity |
| Anti-Hsp70 Immunoassay Plate | 1 Plate |
| Recombinant Hsp70 Standard | 2 vials |
| Standard and Sample Diluent | 1 vial/ 50 ml |
| 10X Wash Buffer Concentrate | 1 vial/100 ml |
| Anti-Hsp70 Biotinylated Antibody Concentrate | 1 vial/150 µl |
| Anti-Hsp70 Biotinylated Antibody Diluent | 1 vial/ 13 ml |
| Streptavidin: HRP Concentrate | 1 vial/150 µl |
| Streptavidin: HRP Diluent | 1 vial/ 13 ml |
| TMB Substrate | 1 vial/ 13 ml |
| Stop Solution | 1 vial/ 13 ml |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Data Example Images
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Typical Standard Curve for the HSP70 ELISA Kit (High-Sensitivity) (Enzyme-Linked Immunosorbent Assay)K7109
Assay Type: Sandwich ELISA.
Detection Method: Colorimetric Assay.
Assay Range: 0.55 35 ng/mL.
Specific Publications For HSP70 ELISA Kit (High-Sensitivity) (EK7109)
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Customer Q&As
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1 Customer Q&As for HSP70 ELISA Kit (High-Sensitivity)
Question
How many tissue samples can be run in a single EK7109 kit?
Verified customer
Asked: 2020-06-19
Answer
The HSP70 ELISA Kit (High-Sensitivity) (EK7109) can run 40 samples in duplicate.
Boster Scientific Support
Answered: 2020-06-22
