Product Info Summary
SKU: | M09275-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Chicken |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Heat Shock Protein 27 Antibody
SKU/Catalog Number
M09275-1
Size
100ul
Description
Boster Bio Anti-Heat Shock Protein 27 Antibody catalog # M09275-1. Tested in IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Heat Shock Protein 27 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09275-1)
Host
Chicken
Contents
Supplied as an aliquot of IgY preparation plus 5mM NaN3
Clonality
Polyclonal
Immunogen
Recombinant full length human HSP27 expressed in and purified from E. coli
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M09275-1 is reactive to HSBP1 in Human
Applications
M09275-1 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
8544 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:5,000. IF/ICC and IHC:1:2,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of whole cell lysates using chicken pAb to HSP27, M09275-1, dilution 1:2,000, in red.
Click image to see more details
Immunofluorescent analysis of HeLa cells stained with chicken pAb to HSP27, M09275-1, dilution 1:1,000 in green, and costained with mouse mAb to HSP60 dilution 1:5,000 in red. Blue is DAPI staining of nuclear DNA. The M09275-1 antibody produces strong cytoplasmic staining, while the HSP60 antibody specifically labels mitochondria.
Protein Target Info & Infographic
Gene/Protein Information For HSBP1 (Source: Uniprot.org, NCBI)
Gene Name
HSBP1
Full Name
Heat shock factor-binding protein 1
Weight
8544 MW
Superfamily
HSBP1 family
Alternative Names
DKFZp686D1664; DKFZp686O24200; heat shock factor binding protein 1; heat shock factor-binding protein 1; HSF1BP; Nasopharyngeal carcinoma-associated antigen 13; NPC-A-13 HSBP1 NPC-A-13 heat shock factor binding protein 1 heat shock factor-binding protein 1|nasopharyngeal carcinoma-associated antigen 13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSBP1, check out the HSBP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSBP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Heat Shock Protein 27 Antibody (M09275-1)
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No publications found for M09275-1
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Customer Q&As
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3 Customer Q&As for Anti-Heat Shock Protein 27 Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-Heat Shock Protein 27 antibody M09275-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-23
Question
We are currently using anti-Heat Shock Protein 27 antibody M09275-1 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-17
Answer
The anti-Heat Shock Protein 27 antibody (M09275-1) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-17
Question
Would anti-Heat Shock Protein 27 antibody M09275-1 work on bovine WB with forebrain?
Verified Customer
Verified customer
Asked: 2017-11-15
Answer
Our lab technicians have not validated anti-Heat Shock Protein 27 antibody M09275-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Heat Shock Protein 27 antibody M09275-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine forebrain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-15