Anti-HDAC-1 (near C-terminus) Antibody

Product Name

Anti-HDAC-1 (near C-terminus) Antibody

View all HDAC1 Antibodies

SKU/Catalog Number

A00256-2

Size

25uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-HDAC-1 (near C-terminus) Antibody (Catalog # A00256-2). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-HDAC-1 (near C-terminus) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00256-2)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.1% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-HDAC-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 450-482 of Human HDAC-1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00256-2 is reactive to HDAC1 in Human

Applications

A00256-2 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

55103 MW

Background of HDAC1

HDAC-1 antibody recognizes HDAC1 (also known as HD1, histone deacetylase 1, RPD3, RPD3L1) which belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IHC: 1:200 - 1:1,000
IF Microscopy: 10ug / ml
WB: 1:1,000 - 1:5,000

Validation Images & Assay Conditions

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Western blot analysis of HDAC1 expression in mouse brain extract (lane 1) and mouse brain extract blocked with peptide (lane 2). HDAC1 at 62KD was detected using rabbit anti-HDAC1 Antigen Affinity purified polyclonal antibody (Catalog # A00256-2) at 1 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Gene/Protein Information For HDAC1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.5.1.98; GON-10; HD1; HD1DKFZp686H12203; Histone Deacetylase 1; KDAC1; reduced potassium dependency, yeast homolog-like 1; RPD3L1; RPD3L1RPD3 HDAC1 GON-10, HD1, KDAC1, RPD3, RPD3L1 histone deacetylase 1 histone deacetylase 1|reduced potassium dependency, yeast homolog-like 1

For more info on HDAC1, check out the HDAC1 Infographic

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In this infographic, you will see the following information for HDAC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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