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Product Info Summary
| SKU: | M00256 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HDAC1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00256
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HDAC1 Rabbit Monoclonal Antibody catalog # M00256. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HDAC1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00256)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AIB-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HDAC1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00256 is reactive to HDAC1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
34 kDa
Calculated molecular weight
55103 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00256 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: human testis tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of HDAC1 expression in (1) C6 cell lysate; (2) NIH/3T3 cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human testis, using HDAC1 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For HDAC1 (Source: Uniprot.org, NCBI)
Gene Name
HDAC1
Full Name
Histone deacetylase 1
Weight
55103 MW
Superfamily
histone deacetylase family
Alternative Names
Histone deacetylase 1;HD1;3.5.1.98;HDAC1;RPD3L1; HDAC1 GON-10, HD1, KDAC1, RPD3, RPD3L1 histone deacetylase 1 histone deacetylase 1|reduced potassium dependency, yeast homolog-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HDAC1, check out the HDAC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HDAC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HDAC1 Rabbit Monoclonal Antibody (M00256)
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No publications found for M00256
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Customer Q&As
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3 Customer Q&As for Anti-HDAC1 Rabbit Monoclonal Antibody
Question
I see that the anti-HDAC1 Rabbit Monoclonal antibody M00256 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-29
Question
We are currently using anti-HDAC1 Rabbit Monoclonal antibody M00256 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
The anti-HDAC1 Rabbit Monoclonal antibody (M00256) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-03
Question
Does anti-HDAC1 Rabbit Monoclonal antibody M00256 work for IHC with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2018-12-10
Answer
According to the expression profile of leukemic t-cell, HDAC1 is highly expressed in leukemic t-cell. So, it is likely that anti-HDAC1 Rabbit Monoclonal antibody M00256 will work for IHC with leukemic t-cell.
Boster Scientific Support
Answered: 2018-12-10
