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Product Info Summary
| SKU: | A01759-4 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Hbegf Antibody Picoband®
SKU/Catalog Number
A01759-4
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Hbegf Antibody Picoband® catalog # A01759-4. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Hbegf Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01759-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Hbegf recombinant protein (Position: D63-L148).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01759-4 is reactive to Hbegf in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
20 kDa
Calculated molecular weight
23.067kDa
Background of HB-EGF
Heparin-binding EGF-like growth factor (HB-EGF) is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene. Enables epidermal growth factor receptor binding activity; growth factor activity; and heparin binding activity. Involved in positive regulation of keratinocyte migration; positive regulation of smooth muscle cell proliferation; and wound healing, spreading of epidermal cells. Acts upstream of or within several processes, including epidermal growth factor receptor signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; and regulation of heart contraction. Located in extracellular space. Is integral component of plasma membrane. Is expressed in several structures, including branchial arch; heart; oral region gland; telencephalon; and tooth. Human ortholog(s) of this gene implicated in glomerulosclerosis and perinatal necrotizing enterocolitis. Orthologous to human HBEGF (heparin binding EGF like growth factor).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01759-4 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat heart tissue, mouse heart tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Hbegf using anti-Hbegf antibody (A01759-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hbegf antigen affinity purified polyclonal antibody (Catalog # A01759-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hbegf at approximately 20 kDa. The expected band size for Hbegf is at 23 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Hbegf (Source: Uniprot.org, NCBI)
Gene Name
Hbegf
Full Name
Proheparin-binding EGF-like growth factor
Weight
23.067kDa
Alternative Names
N6-adenosine-methyltransferase catalytic subunit HBEGF DTR, DTS, DTSF, HEGFL heparin binding EGF like growth factor proheparin-binding EGF-like growth factor|diphtheria toxin receptor (heparin-binding EGF-like growth factor)|diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)|heparin-binding epidermal growth factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Hbegf, check out the Hbegf Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Hbegf: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hbegf Antibody Picoband® (A01759-4)
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