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Product Info Summary
| SKU: | M02393 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Chicken, Cow, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Horse, Xenopus laevis |
| Host: | Rat |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Monoclonal Antibody
View all gp96/HSP90B1/GRP94 Antibodies
SKU/Catalog Number
M02393
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Monoclonal Antibody (Catalog # M02393). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Monkey, Horse, Cow, Sheep, Pig, Rabbit, Dog, Rat, Mouse, Hamster, Guinea pig, Chicken, Xenopus laevis.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02393)
Host
Rat
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM249
Isotype
IgG2a, kappa
Immunogen
Purified glucose regulated protein 94 (grp94) from chicken oviducts
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02393 is reactive to HSP90B1 in Chicken, Cow, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Horse, Xenopus laevis
Reconstitution
Observed Molecular Weight
19 kDa
Calculated molecular weight
92469 MW
Background of gp96/HSP90B1/GRP94
Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This monoclonal antibody is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp's are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02393 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Immunofluorescence analysis of HePG2 cells labeling GRP94 with Anti-GRP94 Monoclonal Antibody (SPM249) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).
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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Anti-GRP94 Monoclonal Antibody (SPM249).
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Flow Cytometric analysis of PFA-fixed HePG2 cells using Anti-GRP94 Monoclonal Antibody (SPM249) followed by Goat anti-Rat- IgG-CF488 (Blue); Isotype Control (Red).
Protein Target Info & Infographic
Gene/Protein Information For HSP90B1 (Source: Uniprot.org, NCBI)
Gene Name
HSP90B1
Full Name
Endoplasmin
Weight
92469 MW
Superfamily
heat shock protein 90 family
Alternative Names
Endoplasmin;94 kDa glucose-regulated protein;GRP-94;Heat shock protein 90 kDa beta member 1;Tumor rejection antigen 1;gp96 homolog;HSP90B1;GRP94, TRA1; HSP90B1 ECGP, GP96, GRP94, HEL-S-125m, HEL35, TRA1 heat shock protein 90 beta family member 1 endoplasmin|94 kDa glucose-regulated protein|endothelial cell (HBMEC) glycoprotein|epididymis luminal protein 35|epididymis secretory sperm binding protein Li 125m|heat shock protein 90 kDa beta member 1|heat shock protein 90kDa beta (Grp94), member 1|heat shock protein 90kDa beta family member 1|stress-inducible tumor rejection gp96|tumor rejection (gp96) 1|tumor rejection 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSP90B1, check out the HSP90B1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSP90B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Monoclonal Antibody (M02393)
Hello CJ!
M02393 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ibutilide protects against cardiomyocytes injury via inhibiting endoplasmic reticulum and mitochondrial stress pathways
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