Product Info Summary
SKU: | A09289 |
---|---|
Size: | 100ug |
Reactive Species: | Mouse |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Ankrd26 Antibody
SKU/Catalog Number
A09289
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Ankrd26 Antibody (Catalog # A09289). Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Ankrd26 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09289)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near amino acids 1-25 of mouse Ankrd26 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09289 is reactive to ANKRD26 in Mouse
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
180647 MW
Background of ANKRD26
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. POTE gene is expressed in prostate and breast cancer and a potential candidate for immuno-therapy. It is also expressed in undifferentiated embryonic stem (ES) cells. ANKRD-26 is the ancestral gene of POTE and has a homologue in mouse. Mouse Ankrd26 is also selectively expressed in ES cells and various cancers but not in other adult tissues. The encoded protein of Ankrd26 contains ankyrin repeats and coiled coil domain of spectrin suggestive of a signaling molecule.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09289 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:20,000 - 1:80,000
WB: 1:500 - 1:3,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Ankrd26 expression in blank (lane 1), MES cell lysate -80
Protein Target Info & Infographic
Gene/Protein Information For ANKRD26 (Source: Uniprot.org, NCBI)
Gene Name
ANKRD26
Full Name
Ankyrin repeat domain-containing protein 26
Weight
180647 MW
Alternative Names
Ankrd26, ankrd 26, ankrd-26, Ankyrin repeat domain 26, Ankyrin repeat domain containing protein 26, bA145E8.1, KIAA1074 ANKRD26 THC2, bA145E8.1 ankyrin repeat domain 26 ankyrin repeat domain-containing protein 26|GNS/ANKRD26/NCKAP1L fusion
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANKRD26, check out the ANKRD26 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANKRD26: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ankrd26 Antibody (A09289)
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No publications found for A09289
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Customer Q&As
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7 Customer Q&As for Anti-Ankrd26 Antibody
Question
I was wanting to use your anti-Ankrd26 antibody for WB for human frontal cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human frontal cortex identification?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
As indicated on the product datasheet, A09289 anti-Ankrd26 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human frontal cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-02
Question
I am looking for to test anti-Ankrd26 antibody A09289 on human frontal cortex for research purposes, then I may be interested in using anti-Ankrd26 antibody A09289 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
The products we sell, including anti-Ankrd26 antibody A09289, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-15
Question
I see that the anti-Ankrd26 antibody A09289 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-01-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-01-23
Question
We are currently using anti-Ankrd26 antibody A09289 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
The anti-Ankrd26 antibody (A09289) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-06
Question
Does anti-Ankrd26 antibody A09289 work for WB with frontal cortex?
Verified Customer
Verified customer
Asked: 2018-07-16
Answer
According to the expression profile of frontal cortex, ANKRD26 is highly expressed in frontal cortex. So, it is likely that anti-Ankrd26 antibody A09289 will work for WB with frontal cortex.
Boster Scientific Support
Answered: 2018-07-16
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for frontal cortex using anti-Ankrd26 antibody A09289. Let me know if you need anything else.
C. Gonzalez
Verified customer
Asked: 2018-01-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-23
Question
Is a blocking peptide available for product anti-Ankrd26 antibody (A09289)?
Z. Patel
Verified customer
Asked: 2015-04-23
Answer
We do provide the blocking peptide for product anti-Ankrd26 antibody (A09289). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-04-23