This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
1 Citations 1 Q&As
1 Citations 7 Q&As
1 Citations
Facts about Endoplasmin.
Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
Human | |
---|---|
Gene Name: | HSP90B1 |
Uniprot: | P14625 |
Entrez: | 7184 |
Belongs to: |
---|
heat shock protein 90 family |
94 kDa glucose-regulated protein; ECGP; Endoplasmin; endothelial cell (HBMEC) glycoprotein; glucose regulated protein, 94 kDa; gp96; GP96ECGP; Grp94; GRP-94; GRP94gp96 homolog; Heat shock protein 90 kDa beta member 1; heat shock protein 90kDa beta (Grp94), member 1; HSP90B1; TRA1endoplasmin; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen-1 (gp96)
Mass (kDA):
92.469 kDA
Human | |
---|---|
Location: | 12q23.3 |
Sequence: | 12; NC_000012.12 (103930410..103947926) |
Endoplasmic reticulum lumen. Sarcoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
The HSP90B1 marker is an important biomarker for many experiments. It helps researchers measure the level of protein expression in cells and their interaction with their surroundings. Despite its importance, it is relatively easy to mishandle. For that reason, Boster Bio provides a wide range of HSP90B1 optimization tips and guides. These guides help researchers decide what controls to set up and how to optimize experiments to improve results. Every researcher runs into trouble during experiments. Troubleshooting guides are an excellent resource for identifying possible sources of error and determining how to avoid them.
The heat shock protein 90kDa beta family member 1 (HSP90B1) is a recombinant protein with the Uniprot ID P14625 and annotated function. This protein is 803 amino acids long and 92.5 kilodaltons in mass. It is encoded by the gene HSP90B1.
The HSP90B1 gene is also known as endoplasmin, GRP94, and sperm binding protein Li 125m. It is a member of the ATP-metabolizing molecular chaperone family. This protein is located on chromosome 12q23.3 and is associated with pathogenic states and tumor formation. It has been found in cancerous cells, and it is a marker of tumor growth.
This boster bio HSP90B1 cell-based ELISPOT kit contains all the reagents necessary to perform a standardized assay. The technique uses antibodies linked with enzymes to quantify the amount of a target molecule. Different formats are available, each with a different principle for antibody-antigen interaction. The Picokine(tm) ELISA kit, for instance, contains antibodies with a high affinity for the native form of the protein. The Boster bio HSP90B1 ELISA kit is also designed to detect this protein in its native form. Boster also validates its kits against relevant superfamilies and similar immunogenic proteins.
The HSP90B1 Cell-Based ELISA Kit can measure the relative amounts of Telomerase in cells. With its high throughput and convenient design, it can also be used to monitor the effects of various treatments or activators on Telomerase protein levels in a variety of samples. The boster Bio HSP90B1 ELISA kit can also monitor the effects of drugs on the production of Thyroid hormones.
To perform the assay, the samples must be diluted appropriately. For this, 100uL of sample should be placed in each micro ELISA plate. Then, the Sample diluent should be added to the blank well. The reagents must be gently mixed without touching the inside walls of the microtiter plate. Sealing the microplates with a sealer is necessary to prevent evaporation of the samples. Incubate the samples at 37degC for 120 minutes. During this time, the Detection Reagent A must be added to each microtiter plate and scanned for colorimetric signals.
The Boster Bio Anti-CD41/Intégrin alpha 2b/ITGA2b antibody was developed by a team of immunologists to target Human Integrin alpha 2b/CD41. The antibodies are highly sensitive and specific for both human and mouse cells. The human antibody is not cross reactive with recombinant Human Integrin alpha 5 or alpha 8 and mouse Immunoglobulin G1 or G2b. For a more accurate result, use this boster bio antibody in ELISAs.
The anti-CD41/Integrin alfa 2b/ITGA2B antibody recognizes fibrinogen, platelets, and plasminogen. The protein's ability to bind fibrinogen gamma chain is critical to platelet aggregation. Inhibition of this protein will lead to platelet detachment and rupture of endothelial cells.
The CD41/ITGA2B antibody has been tested for use in IP and Flow Cytometry. It has been confirmed to recognize CD41 in Mouse samples using Flow Cytometry and immunohistochemistry. If you find that this antibody is ineffective, you can purchase a blocking peptide to block the CD41/Integrin alpha 2b/ITGA2B antibody from Boster Bio. The blocking peptide costs vary depending on the length of the immunogen.
The possibility that the immune system can control tumor growth by modulating the production and release of heat shock proteins (HSPs) is tantalizing. Although the mechanisms by which HSPs enter and exit cells remain unclear, these proteins have similar functions. For example, some are important in the management of chronic diseases while others appear to be tumor vaccines or proliferators. Regardless of their role, HSPs enhance the body's immune system and are crucial in thermotolerance.
While HSPs were initially believed to reside within cancer cells, there are now evidence that they can exit the cell. The soluble HSP 90a (eHSP90) exists outside the cell. It interacts with numerous client proteins to improve the migration and invasion of cancer cells. This mechanism of HSP exocytosis has yet to be fully understood, but is thought to be involved in tumour-promoting inflammation.
Research on HSPs has been focused on cancer, but their role in other diseases is equally intriguing. HSPs have been implicated in neurological disorders, muscle-wasting diseases, obesity, and post-traumatic stress, highlighting the central role of these proteins in cellular functions. Although the role of HSPs in chronic diseases remains unclear, there is a need for further studies to determine whether HSPs have a therapeutic value.
The development of an ELISA kit requires the use of high-affinity antibodies. Several steps are involved in ELISA kit optimization, including the use of a 0.2 um filter, aliquoting supernatants and avoiding freeze-thaw cycles. The next step involves data analysis. Listed below are the steps involved in optimizing an ELISA kit.
For accurate measurement of HSP90B1 in cells and tissues, siRNAs are available in the market. These siRNAs are available in the form of lentivirus, AAV, or oligo. These siRNAs can knock down HSP90B1 in many host cells by transduction and transfection. HSP90B1 siRNA is intended for research purposes only. It is not recommended for therapeutic or diagnostic applications.
PMID: 2377606 by Maki R.G., et al. Human homologue of murine tumor rejection antigen gp96: 5'-regulatory and coding regions and relationship to stress-induced proteins.
PMID: 2546060 by Chang S.C., et al. Glucose-regulated protein (GRP94 and GRP78) genes share common regulatory domains and are coordinately regulated by common trans- acting factors.
*More publications can be found for each product on its corresponding product page