Anti-GRP94 Monoclonal Antibody

Product Name

Anti-GRP94 Monoclonal Antibody

View all gp96/HSP90B1/GRP94 Antibodies

SKU/Catalog Number

M02393-2

Size

200ug

Form

liquid

Description

Boster Bio Anti-GRP94 Monoclonal Antibody catalog # M02393-2. Tested in IP, IF, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GRP94 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02393-2)

Host

Rat

Contents

PBS pH7.2, 50% glycerol, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

9G10

Isotype

IgG2a

Immunogen

Purified Grp94 isolated from chicken oviducts

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~98kDa. Does not detect human HSP90, Grp74, or GrpE from E.coli.

Reactive Species

M02393-2 is reactive to GRP94 in Bovine, Chicken, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus, Horse

Reconstitution

Calculated molecular weight

92.469kDa

Background of gp96/HSP90B1/GRP94

Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2). Grp94 is a member of the HSP90 family of stress proteins and shares sequence homology with its cytosolic equivalent, HSP90 (3). Both HSP90 and Grp94 are calcium binding proteins (4). Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with HSP90, Grp94 and HSP90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolysis activity (5). Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M02393-2 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

Gene/Protein Information For GRP94 (Source: Uniprot.org, NCBI)

Alternative Names

HSP90B1, GP96, TRA1, ECGP, 94 kDa glucose regulated protein, 94 kDa glucose-regulated protein, ECGP, Endoplasmin, Endothelial cell (HBMEC) glycoprotein, ENPL_HUMAN, Glucose regulated protein 94kDa, gp96, gp96 homolog, GRP 94, GRP-94, Heat shock protein 90 kDa beta member 1, heat shock protein 90kDa beta (Grp94), member 1, Heat shock protein, 90 kDa, beta, 1, HSP90B1, Stress inducible tumor rejection antigen GP96, TRA1, tumor rejection antigen (gp96) 1, Tumor rejection antigen 1, Tumor rejection antigen gp96, Tumor rejection antigen-1 (gp96) HSP90B1 ECGP, GP96, GRP94, HEL-S-125m, HEL35, TRA1 heat shock protein 90 beta family member 1 endoplasmin|94 kDa glucose-regulated protein|endothelial cell (HBMEC) glycoprotein|epididymis luminal protein 35|epididymis secretory sperm binding protein Li 125m|heat shock protein 90 kDa beta member 1|heat shock protein 90kDa beta (Grp94), member 1|heat shock protein 90kDa beta family member 1|stress-inducible tumor rejection gp96|tumor rejection (gp96) 1|tumor rejection 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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