Anti-Granzyme M/GZMM Antibody Picoband™

Product Name

Anti-Granzyme M/GZMM Antibody Picoband™

View all Granzyme M Antibodies

SKU/Catalog Number

A08286-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Granzyme M/GZMM Antibody Picoband™ catalog # A08286-1. Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Granzyme M/GZMM Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08286-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Granzyme M/GZMM recombinant protein (Position: A64-D206).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A08286-1 is reactive to GZMM in Mouse

Applications

A08286-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

28 kDa

Calculated molecular weight

Background of Granzyme M

Granzyme M is a protein that in humans is encoded by the GZMM gene. Human natural killer (NK) cells and activated lymphocytes express and store a distinct subset of neutral serine proteases together with proteoglycans and other immune effector molecules in large cytoplasmic granules. These serine proteases are collectively termed granzymes and include 4 distinct gene products: granzyme A, granzyme B, granzyme H, and the protein encoded by this gene, granzyme M. Two transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Mouse
Direct ELISA, 0.1-0.5 μg/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Granzyme M/GZMM using anti-Granzyme M/GZMM antibody (A08286-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse SP2/0 whole cell lysates,
Lane 2: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Granzyme M/GZMM antigen affinity purified polyclonal antibody (Catalog # A08286-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Granzyme M/GZMM at approximately 28 kDa. The expected band size for Granzyme M/GZMM is at 28 kDa.

Gene/Protein Information For GZMM (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.4.21; EC 3.4.21.-; granzyme M (lymphocyte met-ase 1); granzyme M; Hu-Met-1; LMET1; lymphocyte met-ase 1; Met-1 serine protease; MET1met-ase; Met-ase; Natural killer cell granular protease GZMM LMET1, MET1 granzyme M granzyme M|HU-Met-1|Met-1 serine protease|lymphocyte met-ase 1|met-ase|natural killer cell granular protease

For more info on GZMM, check out the GZMM Infographic

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In this infographic, you will see the following information for GZMM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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