Anti-Granzyme B GZMB Antibody

Product Name

Anti-Granzyme B GZMB Antibody

View all Granzyme B Antibodies

SKU/Catalog Number

A00353

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Granzyme B GZMB Antibody (Catalog # A00353). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Granzyme B GZMB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00353)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Granzyme B.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00353 is reactive to GZMB in Human, Mouse, Rat

Reconstitution

Calculated molecular weight

27716 MW

Background of Granzyme B

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

David A. Jans, J. Biol. Chem., Nov 1996; 271: 30781.
Eugene Estella, Diabetes, Aug 2006; 55: 2212 - 2219.
Yong Gu, J. Biol. Chem., May 1996; 271: 10816.
Jiuru Sun, J. Biol. Chem., Nov 1996; 271: 27802.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A00353 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For GZMB (Source: Uniprot.org, NCBI)

Alternative Names

Granzyme B;3.4.21.79;C11;CTLA-1;Cathepsin G-like 1;CTSGL1;Cytotoxic T-lymphocyte proteinase 2;Lymphocyte protease;Fragmentin-2;Granzyme-2;Human lymphocyte protein;HLP;SECT;T-cell serine protease 1-3E;GZMB;CGL1, CSPB, CTLA1, GRB; GZMB C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT granzyme B granzyme B|T-cell serine protease 1-3E|cathepsin G-like 1|cytotoxic T-lymphocyte proteinase 2|cytotoxic serine protease B|fragmentin 2|granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)|human lymphocyte protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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