Anti-Nucleolysin TIA-1 isoform p40 TIA1 Antibody Picoband®

Product Name

Anti-Nucleolysin TIA-1 isoform p40 TIA1 Antibody Picoband®

View all TIA1 Antibodies

SKU/Catalog Number

PA2194

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Nucleolysin TIA-1 isoform p40 TIA1 Antibody catalog # PA2194. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Nucleolysin TIA-1 isoform p40 TIA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2194)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human TIA1, identical to the related mouse sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2194 is reactive to TIA1 in Human, Mouse

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

274 kDa

Calculated molecular weight

42963 MW

Background of TIA1

TIA1, also called WDM, encodes an RNA-binding protein involved in splicing regulation and translational repression. The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. By in situ hybridization, this gene is mapped to chromosome 2p13.3. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly (A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is though to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2194 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse

Positive Control

WB: JURKAT Cell, RAJI Cell, CEM Cell, HT1080 Cell, K562 Cell,

Validation Images & Assay Conditions

Gene/Protein Information For TIA1 (Source: Uniprot.org, NCBI)

Alternative Names

Nucleolysin TIA-1 isoform p40;RNA-binding protein TIA-1;T-cell-restricted intracellular antigen-1;TIA-1;p40-TIA-1;TIA1; TIA1 ALS26, TIA-1, WDM TIA1 cytotoxic granule associated RNA binding protein nucleolysin TIA-1 isoform p40|nucleolysin TIA-1|T-cell-restricted intracellular -1|p40-TIA-1 (containing p15-TIA-1)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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