Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | M00898 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-GNAQ Antibody Picoband® (monoclonal, 13H4)
SKU/Catalog Number
M00898
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GNAQ Antibody Picoband® (monoclonal, 13H4) catalog # M00898. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GNAQ Antibody Picoband® (monoclonal, 13H4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00898)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
13H4
Isotype
Mouse IgG2b
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human GNAQ, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00898 is reactive to GNAQ in Human, Monkey, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
42.142kDa
Background of GNAQ
Guanine nucleotide-binding protein G (q) subunit alpha is a protein that in humans is encoded by the GNAQ gene. Guanine nucleotide-binding proteins are a family of heterotrimeric proteins that couple cell surface, 7-transmembrane domain receptors to intracellular signaling pathways. Receptor activation catalyzes the exchange of GDP for GTP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit. G-alpha-q is the alpha subunit of one of the heterotrimeric GTP-binding proteins that mediates stimulation of phospholipase C-beta. Mutations in this gene have been found associated to cases of Sturge-Weber syndrome and port-wine stains.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00898 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
WB: human Jurkat whole cell, human Hela whole cell, human A549 whole cellhuman A431 whole cell, human MCF-7 whole cell, human K562 whole cell, monkey COS-7 whole cell, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue
IHC: human ovarian cancer tissue, human ovarian cancer tissue, mouse testis tissue
FCM: U20S cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GNAQ using anti-GNAQ antibody (M00898).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A549 whole cell lysates.
Lane 4: human A431 whole cell lysates,
Lane 5: human MCF-7 whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: monkey COS-7 whole cell lysates,
Lane 8: rat brain tissue lysates,
Lane 9: rat lung tissue lysates,
Lane 10: mouse brain tissue lysates,
Lane 11: mouse lung tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GNAQ antigen affinity purified monoclonal antibody (Catalog # M00898) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GNAQ at approximately 42KD. The expected band size for GNAQ is at 42KD.
Click image to see more details
Figure 2. IHC analysis of GNAQ using anti GNAQ antibody (M00898).
GNAQ was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GNAQ Antibody (M00898) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of GNAQ using anti GNAQ antibody (M00898).
GNAQ was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GNAQ Antibody (M00898) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of GNAQ using anti GNAQ antibody (M00898).
GNAQ was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GNAQ Antibody (M00898) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 5. Flow Cytometry analysis of U20S cells using anti- GNAQ antibody (M00898).
Overlay histogram showing U20S cells stained with M00898 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GNAQ Antibody (M00898, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For GNAQ (Source: Uniprot.org, NCBI)
Gene Name
GNAQ
Full Name
Guanine nucleotide-binding protein G(q) subunit alpha
Weight
42.142kDa
Superfamily
G-alpha family
Alternative Names
Guanine nucleotide-binding protein G (q) subunit alpha; Guanine nucleotide-binding protein alpha-q; GNAQ; GAQ GNAQ CMC1, G-ALPHA-q, GAQ, SWS G protein subunit alpha q guanine nucleotide-binding protein G(q) subunit alpha|epididymis secretory sperm binding protein|guanine nucleotide binding protein (G protein), q polypeptide|guanine nucleotide-binding protein alpha-q
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GNAQ, check out the GNAQ Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GNAQ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GNAQ Antibody Picoband® (monoclonal, 13H4) (M00898)
Hello CJ!
No publications found for M00898
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-GNAQ Antibody Picoband® (monoclonal, 13H4)?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-GNAQ Antibody Picoband® (monoclonal, 13H4)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-GNAQ Antibody Picoband® (monoclonal, 13H4)
Question
Is there a BSA free version of anti-GNAQ antibody (monoclonal, 13H4) M00898 available?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GNAQ antibody (monoclonal, 13H4) M00898 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-16
Question
I am interested in to test anti-GNAQ antibody (monoclonal, 13H4) M00898 on human thoracic mammary gland for research purposes, then I may be interested in using anti-GNAQ antibody (monoclonal, 13H4) M00898 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Carter
Verified customer
Asked: 2019-11-22
Answer
The products we sell, including anti-GNAQ antibody (monoclonal, 13H4) M00898, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-22
Question
I have a question about product M00898, anti-GNAQ antibody (monoclonal, 13H4). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00898 anti-GNAQ antibody (monoclonal, 13H4), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-20
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thoracic mammary gland using anti-GNAQ antibody (monoclonal, 13H4) M00898. Let me know if you need anything else.
D. Krishna
Verified customer
Asked: 2018-11-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-27
Question
Is a blocking peptide available for product anti-GNAQ antibody (monoclonal, 13H4) (M00898)?
G. Huang
Verified customer
Asked: 2018-08-07
Answer
We do provide the blocking peptide for product anti-GNAQ antibody (monoclonal, 13H4) (M00898). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-08-07
Question
I was wanting to use your anti-GNAQ antibody (monoclonal, 13H4) for Flow Cytometry for human thoracic mammary gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human thoracic mammary gland identification?
Verified Customer
Verified customer
Asked: 2017-07-31
Answer
It shows on the product datasheet, M00898 anti-GNAQ antibody (monoclonal, 13H4) has been validated for Flow Cytometry, IHC-P, WB on human, monkey tissues. We have an innovator award program that if you test this antibody and show it works in human thoracic mammary gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-31
