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Product Info Summary
| SKU: | EK1121 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human TNFRSF13C/BAFFR ELISA Kit PicoKine®
View all BAFFR/TNFRSF13C ELISA kits
SKU/Catalog Number
EK1121
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TNFRSF13C/BAFFR ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF13C in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TNFRSF13C/BAFFR ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1121)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: S7-A71
Sensitivity
<10 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1121 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1121 is reactive to TNFRSF13C in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1121 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of BAFFR/TNFRSF13C
Tumor necrosis factor receptor superfamily member 13C also known as B-cell-activating factor receptor or BAFF receptor is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1121-CAP | Anti-Human TNFRSF13C Pre-coated 96-well strip microplate | 1 |
| EK1121-ST | Human TNFRSF13C Standard | 2 vials, 10 ng/tube |
| EK1121-DA | Human TNFRSF13C Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.044 | 0.218 | 0.341 | 0.539 | 0.837 | 1.245 | 1.699 | 2.012 |
Data Example Images
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Human TNFRSF13C/BAFFR PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect BAFFR/TNFRSF13C, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 78 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TNFRSF13C/BAFFR ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 147 | 683 | 2750 | 156 | 700 | 2531 |
| Standard deviation | 10.29 | 49.85 | 206.25 | 13.88 | 62.3 | 227.05 |
| CV (%) | 7% | 7.3% | 7.5% | 8.9% | 8.9% | 8.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of BAFFR/TNFRSF13C in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 147 | 162 | 135 | 138 | 145 | 10.5 | 7.2% |
| Sample 2 | 683 | 747 | 709 | 652 | 697 | 34.86 | 5% |
| Sample 3 | 2750 | 3116 | 2752 | 2808 | 2856 | 151.62 | 5.3% |
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF13C (Source: Uniprot.Org, NCBI)
Gene Name
TNFRSF13C
Full Name
Tumor necrosis factor receptor superfamily member 13C
Weight
18864 MW
Alternative Names
Tumor necrosis factor receptor superfamily member 13C;B-cell-activating factor receptor;BAFF receptor;BAFF-R;BLyS receptor 3;CD268;TNFRSF13C;BAFFR, BR3; Tnfrsf13c|2010006P15Rik, BAFF, BAFF-R, Baf, Baffr, Bcmd, Bcmd-1, Bcmd1, Lv, Lvis22|tumor necrosis factor receptor superfamily, member 13c|tumor necrosis factor receptor superfamily member 13C|B-cell maturation defect 1|B-cell-activating factor receptor|BAFF receptor|BLyS receptor 3|b cell-activating factor receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TNFRSF13C, check out the TNFRSF13C Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFRSF13C: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TNFRSF13C/BAFFR ELISA Kit PicoKine® (EK1121)
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Customer Q&As
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6 Customer Q&As for Human TNFRSF13C/BAFFR ELISA Kit PicoKine®
Question
I want to purchase the Human TNFRSF13C/BAFFR ELISA Kit PicoKine, I wonder whether this antibody can detect my mutated BFFR protein. Would you tell me which amino acids sequence of the epitope in human BAFFR?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
The peptide sequence used to generate this Human TNFRSF13C/BAFFR ELISA Kit PicoKine antibody is GSTELVTTKTAGPEQ and the amino acids is 169-183 human BAFFR receptor.
Boster Scientific Support
Answered: 2020-04-22
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-03-06
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
H. Sunder
Verified customer
Asked: 2020-02-15
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-02-15
Question
Q: can I use citrate plasma as samples in Human BAFFR Picokine® ELISA Kit (Catalog # EK1121)?
Verified Customer
Verified customer
Asked: 2018-08-18
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of BAFFR causing degradation of its protein structure. BAFFR may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the BAFFR ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-08-18
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement BAFFR using the EK1121 Human BAFFR Picokine® ELISA Kit?
B. Martin
Verified customer
Asked: 2018-02-22
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2018-02-22
Question
Q: how can I thaw whole blood sample for BAFFR ELISA after freezing?
P. Varma
Verified customer
Asked: 2015-12-03
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test BAFFR for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2015-12-03
