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Product Info Summary
| SKU: | A02884-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Glycerol kinase/GK Antibody Picoband®
View all Glycerol Kinase Antibodies
SKU/Catalog Number
A02884-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Glycerol kinase/GK Antibody Picoband® catalog # A02884-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glycerol kinase/GK Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02884-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Glycerol kinase recombinant protein (Position: M1-S180).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02884-1 is reactive to GK in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
61 kDa
Calculated molecular weight
61.245kDa
Background of Glycerol Kinase
Glycerol kinase is a phosphotransferase enzyme involved in triglycerides and glycerophospholipids synthesis. The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02884-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human placenta tissue, human HepG2 whole cell, human SW579 whole cell, human SK-OV-3 whole cell, human U-87MG whole cell, rat testis tissue
IHC: mouse kidney tissue, rat kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Glycerol kinase using anti-Glycerol kinase antibody (A02884-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human SW579 whole cell lysates,
Lane 4: human SK-OV-3 whole cell lysates,
Lane 5: human U-87MG whole cell lysates,
Lane 6: rat testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glycerol kinase antigen affinity purified polyclonal antibody (Catalog # A02884-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glycerol kinase at approximately 61KD. The expected band size for Glycerol kinase is at 61KD.
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Figure 2. IHC analysis of Glycerol kinase using anti-Glycerol kinase antibody (A02884-1).
Glycerol kinase was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Glycerol kinase Antibody (A02884-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of Glycerol kinase using anti-Glycerol kinase antibody (A02884-1).
Glycerol kinase was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Glycerol kinase Antibody (A02884-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For GK (Source: Uniprot.org, NCBI)
Gene Name
GK
Full Name
Glycerol kinase
Weight
61.245kDa
Superfamily
FGGY kinase family
Alternative Names
Glycerol kinase; GK; Glycerokinase; ATP:glycerol 3-phosphotransferase; GK GK GK1D, GK glycerol kinase glycerol kinase|ATP:glycerol 3-phosphotransferase|glycerokinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GK, check out the GK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Glycerol kinase/GK Antibody Picoband® (A02884-1)
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Customer Q&As
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4 Customer Q&As for Anti-Glycerol kinase/GK Antibody Picoband®
Question
We are currently using anti-Glycerol kinase/GK antibody A02884-1 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
The anti-Glycerol kinase/GK antibody (A02884-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-21
Question
Does A02884-1 anti-Glycerol kinase/GK antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
It shows on the product datasheet, A02884-1 anti-Glycerol kinase/GK antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-09
Question
Please see the WB image, lot number and protocol we used for adrenal tissue using anti-Glycerol kinase/GK antibody A02884-1. Please let me know if you require anything else.
P. Li
Verified customer
Asked: 2018-03-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-09
Question
Do you have a BSA free version of anti-Glycerol kinase/GK antibody A02884-1 available?
C. Evans
Verified customer
Asked: 2015-07-22
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Glycerol kinase/GK antibody A02884-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-07-22
