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Product Info Summary
| SKU: | A03572 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC |
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Product info
Product Name
Anti-PLC-g2(Ab-1217) Antibody
View all PLC-gamma 2 Antibodies
SKU/Catalog Number
A03572
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-PLC-g2(Ab-1217) Antibody (Catalog # A03572). Tested in IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PLC-g2(Ab-1217) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03572)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.1215~1219 (F-L-Y-D-T) derived from Human PLCg2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03572 is reactive to PLCG2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
147870 MW
Background of PLC-gamma 2
The production of the second messenger molecules diacylglycerol. (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Singer, W. D. et al. (1997) Annu. Rev. Biochem. 66, 475-509.
Smrcka, A. V. et al. (1991) Science 251, 804-807.
Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225.
Watanabe, D. et al. (2001) J. Biol. Chem. 276, 38595-38601.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03572 is guaranteed for IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 150kd
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PLC-g2(Ab-1217) antibody A03572(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using PLC-g2(Ab-1217) antibody A03572.
Protein Target Info & Infographic
Gene/Protein Information For PLCG2 (Source: Uniprot.org, NCBI)
Gene Name
PLCG2
Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
Weight
147870 MW
Alternative Names
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2;3.1.4.11;Phosphoinositide phospholipase C-gamma-2;Phospholipase C-IV;PLC-IV;Phospholipase C-gamma-2;PLC-gamma-2;PLCG2; PLCG2 APLAID, FCAS3, PLC-IV, PLC-gamma-2 phospholipase C gamma 2 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2|phosphoinositide phospholipase C-gamma-2|phospholipase C, gamma 2 (phosphatidylinositol-specific)|phospholipase C-IV
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLCG2, check out the PLCG2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLCG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PLC-g2(Ab-1217) Antibody (A03572)
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No publications found for A03572
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-PLC-g2(Ab-1217) Antibody
Question
See attached the WB image, lot number and protocol we used for spleen using anti-PLC-g2(Ab-1217) antibody A03572. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-22
Question
Is this A03572 anti-PLC-g2(Ab-1217) antibody reactive to the isotypes of PLCG2?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
The immunogen of A03572 anti-PLC-g2(Ab-1217) antibody is Peptide sequence around aa.1215~1219 (F-L-Y-D-T) derived from Human PLCg2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-07
Question
I see that the anti-PLC-g2(Ab-1217) antibody A03572 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-21
Question
Does A03572 anti-PLC-g2(Ab-1217) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
It shows on the product datasheet, A03572 anti-PLC-g2(Ab-1217) antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-13
Question
I was wanting to use your anti-PLC-g2(Ab-1217) antibody for IHC for human spleen on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human spleen identification?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
As indicated on the product datasheet, A03572 anti-PLC-g2(Ab-1217) antibody has been tested for IF, IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human spleen in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-08
Question
My question regarding product A03572, anti-PLC-g2(Ab-1217) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03572 anti-PLC-g2(Ab-1217) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-31
Question
Is there a BSA free version of anti-PLC-g2(Ab-1217) antibody A03572 available?
Verified Customer
Verified customer
Asked: 2018-09-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PLC-g2(Ab-1217) antibody A03572 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-19
Question
We are currently using anti-PLC-g2(Ab-1217) antibody A03572 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-17
Answer
The anti-PLC-g2(Ab-1217) antibody (A03572) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-17
Question
We have been able to see staining in rat lymphoblast. Do you have any suggestions? Is anti-PLC-g2(Ab-1217) antibody supposed to stain lymphoblast positively?
Verified Customer
Verified customer
Asked: 2018-03-15
Answer
From literature lymphoblast does express PLCG2. From Uniprot.org, PLCG2 is expressed in renal glomerulus, lymphoblast, spleen, lymph, leukemic t-cell, among other tissues. Regarding which tissues have PLCG2 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymph, Pubmed ID: 15489334
Lymphoblast, Pubmed ID: 2849563
Spleen, Pubmed ID: 16533400
Boster Scientific Support
Answered: 2018-03-15
Question
We want to test anti-PLC-g2(Ab-1217) antibody A03572 on human spleen for research purposes, then I may be interested in using anti-PLC-g2(Ab-1217) antibody A03572 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-11-08
Answer
The products we sell, including anti-PLC-g2(Ab-1217) antibody A03572, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-08
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for spleen using anti-PLC-g2(Ab-1217) antibody A03572. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-06-30
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-30
Question
Our lab want to know about using your anti-PLC-g2(Ab-1217) antibody for regulation of gene expression studies. Has this antibody been tested with western blotting on lung carcinoma tissue? We would like to see some validation images before ordering.
J. Krishna
Verified customer
Asked: 2015-07-14
Answer
Thank you for your inquiry. This A03572 anti-PLC-g2(Ab-1217) antibody is tested on lung carcinoma tissue, hela cells. It is guaranteed to work for IF, IHC in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-07-14
Question
Would anti-PLC-g2(Ab-1217) antibody A03572 work for IHC with spleen?
N. Baker
Verified customer
Asked: 2015-02-18
Answer
According to the expression profile of spleen, PLCG2 is highly expressed in spleen. So, it is likely that anti-PLC-g2(Ab-1217) antibody A03572 will work for IHC with spleen.
Boster Scientific Support
Answered: 2015-02-18
Question
Is a blocking peptide available for product anti-PLC-g2(Ab-1217) antibody (A03572)?
A. Mangal
Verified customer
Asked: 2013-07-15
Answer
We do provide the blocking peptide for product anti-PLC-g2(Ab-1217) antibody (A03572). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-07-15
Question
We purchased anti-PLC-g2(Ab-1217) antibody for IHC on lymph in the past. I am using human, and We are going to use the antibody for IF next. We want examining lymph as well as spleen in our next experiment. Could give a recommendation on which antibody would work the best for IF?
E. Bhatt
Verified customer
Asked: 2013-03-06
Answer
I have checked the website and datasheets of our anti-PLC-g2(Ab-1217) antibody and it seems that A03572 has been validated on human in both IHC and IF. Thus A03572 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-03-06
