Product Info Summary
SKU: | M03191 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-Glutamine Synthetase GLUL Rabbit Monoclonal Antibody
View all Glutamine Synthetase Antibodies
SKU/Catalog Number
M03191
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Glutamine Synthetase GLUL Rabbit Monoclonal Antibody catalog # M03191. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glutamine Synthetase GLUL Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03191)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CCH-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Glutamine Synthetase
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03191 is reactive to GLUL in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
35 kDa
Calculated molecular weight
42064 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03191 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
FC 1:50
Positive Control
IHC: Human glioblastoma tissue, Human prostate cancer tissue, Rat kidney tissue, Mouse ovary tissue, Rat cerebral cortex tissue, human liver tissue
Validation Images & Assay Conditions
![m03191 wb7 m03191 wb7](https://www.bosterbio.com/media/catalog/product/m/0/m03191-wb7.jpg)
Click image to see more details
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
![m03191 wb m03191 wb](https://www.bosterbio.com/media/catalog/product/m/0/m03191-wb.jpg)
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Western blot analysis of Glutamine Synthetase expression in (1)Jurkat cell lysate;(2) HeLa cell lysate.
![m03191 ihc9 m03191 ihc9](https://www.bosterbio.com/media/catalog/product/m/0/m03191-ihc9.jpg)
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using the Antibody at 1:250 dilution.
![m03191 ihc10 m03191 ihc10](https://www.bosterbio.com/media/catalog/product/m/0/m03191-ihc10.jpg)
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:250 dilution.
![m03191 ihc7 m03191 ihc7](https://www.bosterbio.com/media/catalog/product/m/0/m03191-ihc7.jpg)
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Rat kidney, using the Antibody at 1:400 dilution.
![m03191 ihc11 m03191 ihc11](https://www.bosterbio.com/media/catalog/product/m/0/m03191-ihc11.jpg)
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Mouse ovary, using the Antibody at 1:400 dilution.
![m03191 ihc8 m03191 ihc8](https://www.bosterbio.com/media/catalog/product/m/0/m03191-ihc8.jpg)
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex, using the Antibody at 1:400 dilution.
![m03191 ihc m03191 ihc](https://www.bosterbio.com/media/catalog/product/m/0/m03191-ihc.jpg)
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human liver, using Glutamine Synthetase Antibody .
![m03191 icc1 m03191 icc1](https://www.bosterbio.com/media/catalog/product/m/0/m03191-icc1.jpg)
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For GLUL (Source: Uniprot.org, NCBI)
Gene Name
GLUL
Full Name
Glutamine synthetase
Weight
42064 MW
Superfamily
glutamine synthetase family
Alternative Names
Glutamine synthetase;GS;6.3.1.2;Glutamate decarboxylase;4.1.1.15;Glutamate--ammonia ligase;GLUL;GLNS; GLUL GLNS, GS, PIG43, PIG59 glutamate-ammonia ligase glutamine synthetase|cell proliferation-inducing protein 59|glutamate decarboxylase|glutamine synthase|palmitoyltransferase GLUL|proliferation-inducing protein 43
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GLUL, check out the GLUL Infographic
![GLUL infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GLUL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Glutamine Synthetase GLUL Rabbit Monoclonal Antibody (M03191)
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Customer Q&As
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3 Customer Q&As for Anti-Glutamine Synthetase GLUL Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-Glutamine Synthetase Rabbit Monoclonal antibody for IHC for human muscle testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human muscle testis identification?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
As indicated on the product datasheet, M03191 anti-Glutamine Synthetase Rabbit Monoclonal antibody has been tested for IP, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human muscle testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-31
Question
I see that the anti-Glutamine Synthetase Rabbit Monoclonal antibody M03191 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-29
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-03-29
Question
We are currently using anti-Glutamine Synthetase Rabbit Monoclonal antibody M03191 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
R. Roberts
Verified customer
Asked: 2016-07-28
Answer
The anti-Glutamine Synthetase Rabbit Monoclonal antibody (M03191) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-07-28