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Product Info Summary
| SKU: | A01134-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Factor D/Cfd Antibody Picoband®
View all Complement Factor D/Adipsin Antibodies
SKU/Catalog Number
A01134-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Factor D/Cfd Antibody Picoband® catalog # A01134-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Factor D/Cfd Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01134-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat Factor D recombinant protein (Position: I26-A263). Mouse Factor D shares 69% and 84.5% amino acid (aa) sequence identity with human and rat Factor D, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01134-2 is reactive to Cfd in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
27 kDa
Calculated molecular weight
27.033kDa
Background of Complement Factor D/Adipsin
Factor D is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene is a member of the trypsin family of peptidases. The encoded protein is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. It is also a serine protease that is secreted by adipocytes into the bloodstream. And it stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01134-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat RH35 cell, rat PC-12 cell
IHC: mouse lung tissue, rat lung tissue, rat small intestine tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Factor D using anti-Factor D antibody (A01134-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat RH35 cell lysates,
Lane 2: rat PC-12 cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor D antigen affinity purified polyclonal antibody (Catalog # A01134-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor D at approximately 27KD. The expected band size for Factor D is at 27KD.
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Figure 2. IHC analysis of Factor D using anti-Factor D antibody (A01134-2).
Factor D was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Factor D Antibody (A01134-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of Factor D using anti-Factor D antibody (A01134-2).
Factor D was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Factor D Antibody (A01134-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Factor D using anti-Factor D antibody (A01134-2).
Factor D was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Factor D Antibody (A01134-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Cfd (Source: Uniprot.org, NCBI)
Gene Name
Cfd
Full Name
Complement factor D
Weight
27.033kDa
Superfamily
peptidase S1 family
Alternative Names
Complement factor D; Adipsin; C3 convertase activator; Endogenous vascular elastase; Properdin factor D; Cfd; Adn; Df CFD ADIPSIN, ADN, DF, PFD complement factor D complement factor D|C3 convertase activator|D component of complement (adipsin)|complement factor D preproprotein|properdin factor D
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cfd, check out the Cfd Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cfd: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Factor D/Cfd Antibody Picoband® (A01134-2)
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