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Product Info Summary
| SKU: | PB9214 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HNF1/HNF1A Antibody Picoband®
SKU/Catalog Number
PB9214
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HNF1/HNF1A Antibody Picoband® catalog # PB9214. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HNF1/HNF1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9214)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HNF1 recombinant protein (Position: A431-Q631). Human HNF1 shares 93% and 92% amino acid (aa) sequences identity with mouse and rat HNF1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9214 is reactive to HNF1A in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
80 kDa
Calculated molecular weight
67356 MW
Background of HNF1
HNF1 homeobox A (hepatocyte nuclear factor 1 homeobox A), also known as HNF1A or TCF1, is a human gene. It is mapped to chromosome 12q24.3. The protein encoded by this gene is a transcription factor that is highly expressed in the liver and is involved in the regulation of the expression of several liver-specific genes. HNF1A can function as a dimer. What’s more, deletion of the HNF1A activation domains or interruption of the HNF1-binding sites in the LCT promoter resulted in complete loss of transcriptional activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9214 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human HepG2 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HNF1/HNF1A using anti-HNF1/HNF1A antibody (PB9214).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNF1/HNF1A antigen affinity purified polyclonal antibody (Catalog # PB9214) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HNF1/HNF1A at approximately 80 kDa. The expected band size for HNF1/HNF1A is at 67 kDa.
Protein Target Info & Infographic
Gene/Protein Information For HNF1A (Source: Uniprot.org, NCBI)
Gene Name
HNF1A
Full Name
Hepatocyte nuclear factor 1-alpha
Weight
67356 MW
Superfamily
HNF1 homeobox family
Alternative Names
Hepatocyte nuclear factor 1-alpha;HNF-1-alpha;HNF-1A;Liver-specific transcription factor LF-B1;LFB1;Transcription factor 1;TCF-1;HNF1A;TCF1; HNF1A HNF-1A, HNF1, HNF1alpha, HNF4A, IDDM20, LFB1, MODY3, TCF-1, TCF1 HNF1 homeobox A hepatocyte nuclear factor 1-alpha|HNF-1-alpha|albumin proximal factor|hepatic nuclear factor 1|interferon production regulator factor|liver-specific transcription factor LF-B1|transcription factor 1, hepatic|truncated hepatocyte nuclear factor 1 alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HNF1A, check out the HNF1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HNF1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HNF1/HNF1A Antibody Picoband® (PB9214)
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1 Customer Q&As for Anti-HNF1/HNF1A Antibody Picoband®
Question
We are currently using anti-HNF1/HNF1A antibody PB9214 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on zebrafish tissues as well?
T. Jones
Verified customer
Asked: 2018-07-23
Answer
The anti-HNF1/HNF1A antibody (PB9214) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-23
