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Product Info Summary
| SKU: | PA1303 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-S100 beta/S100B Antibody Picoband®
SKU/Catalog Number
PA1303
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-S100 beta/S100B Antibody catalog # PA1303. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-S100 beta/S100B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1303)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human S100 beta, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1303 is reactive to S100B in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
11 kDa
Calculated molecular weight
10713 MW
Background of S100B
S100 calcium binding protein B or S100B is a protein of the S-100 protein family. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. S100B is a glial-derived protein that is a well-established biomarker for severity of neurological injury and prognosis for recovery. S100 beta is a calcium-binding protein that is expressed at high levels in brain primarily by astrocytes. Addition of the disulfide-bonded dimeric form of S100 beta to primary neuronal and glial cultures and established cell lines induces axonal extension and alterations in astrocyte proliferation and phenotype, but evidence that S100 beta exerts the same effects in vivo has not been presented. Reeves et al. (1994) demonstrated that the same effects of the S100B protein are exerted in vivo. They found that both astrocytosis and neurite proliferation occurred in transgenic mice expressing elevated levels of S100b. They suggested that these transgenic mice represent a useful model for studies of the role of S100B in glial-neuronal interactions in normal development and function of the brain and for analyzing the significance of elevated levels of the protein in Down syndrome and Alzheimer disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1303 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Brain Tissue, Rat Brain Tissue, MCF-7 Cell, HELA Cell, SMMC Cell, JURKAT Cell, COLO320 Cell
Validation Images & Assay Conditions
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Anti-S100 beta antibody, PA1303, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: JURKAT Cell Lysate
Lane 7: COLO320 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For S100B (Source: Uniprot.org, NCBI)
Gene Name
S100B
Full Name
Protein S100-B
Weight
10713 MW
Superfamily
S-100 family
Alternative Names
Protein S100-B;S-100 protein beta chain;S-100 protein subunit beta;S100 calcium-binding protein B;S100B; S100B NEF, S100, S100-B, S100beta S100 calcium binding protein B protein S100-B|S-100 calcium-binding protein, beta chain|S-100 protein subunit beta|S100 calcium-binding protein, beta (neural)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on S100B, check out the S100B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for S100B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-S100 beta/S100B Antibody Picoband® (PA1303)
Hello CJ!
PA1303 has been cited in 21 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Baicalin promotes the viability of Schwann cells in vitro by regulating neurotrophic factors
Expression of S100B during the innate immune of corneal epithelium against fungi invasion
A metanephric adenoma of the kidney associated with polycythemia: A case report
Dendritic cells and macrophages neurally hard-wired in the lymph node
Up-regulation of P2X7 receptors mediating proliferation of Schwann cells after sciatic nerve injury
Graft of a Tissue-Engineered Neural Scaffold Serves as a Promising Strategy to Restore Myelination after Rat Spinal Cord Transection
Inhibition of HMGB1 reduces rat spinal cord astrocytic swelling and AQP4 expression after oxygen-glucose deprivation and reoxygenation via TLR4 and NF-?B signaling in an IL-6-dependent manner
Pro?neurogenic effects of andrographolide on RSC96 Schwann cells in?vitro
Different methods for inducing adipose-derived stem cells to differentiate into Schwann-like cells
Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-S100 beta/S100B Antibody Picoband®
Question
I see that the anti-S100 beta/S100B antibody PA1303 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-08
Question
I was wanting to use your anti-S100 beta/S100B antibody for WB for rat skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat skin identification?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
As indicated on the product datasheet, PA1303 anti-S100 beta/S100B antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-04
Question
I have attached the WB image, lot number and protocol we used for skin using anti-S100 beta/S100B antibody PA1303. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-19
Question
My question regarding product PA1303, anti-S100 beta/S100B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1303 anti-S100 beta/S100B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-10
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin using anti-S100 beta/S100B antibody PA1303. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-09
Question
Is this PA1303 anti-S100 beta/S100B antibody reactive to the isotypes of S100B?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
The immunogen of PA1303 anti-S100 beta/S100B antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human S100 beta(64-78aa DGDGECDFQEFMAFV), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-22
Question
Our lab want to know about to test anti-S100 beta/S100B antibody PA1303 on rat skin for research purposes, then I may be interested in using anti-S100 beta/S100B antibody PA1303 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
The products we sell, including anti-S100 beta/S100B antibody PA1303, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-20
Question
Will anti-S100 beta/S100B antibody PA1303 work for WB with skin?
N. Krishna
Verified customer
Asked: 2019-07-29
Answer
According to the expression profile of skin, S100B is highly expressed in skin. So, it is likely that anti-S100 beta/S100B antibody PA1303 will work for WB with skin.
Boster Scientific Support
Answered: 2019-07-29
Question
We are currently using anti-S100 beta/S100B antibody PA1303 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
The anti-S100 beta/S100B antibody (PA1303) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-12
Question
We have observed staining in rat c1 segment of cervical spinal cord. Are there any suggestions? Is anti-S100 beta/S100B antibody supposed to stain c1 segment of cervical spinal cord positively?
M. Parker
Verified customer
Asked: 2019-06-10
Answer
From literature c1 segment of cervical spinal cord does express S100B. From Uniprot.org, S100B is expressed in c1 segment of cervical spinal cord, skin, among other tissues. Regarding which tissues have S100B expression, here are a few articles citing expression in various tissues:
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-06-10
Question
We are interested in using your anti-S100 beta/S100B antibody for traf6 mediated nf-kb activation studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-01-02
Answer
Thanks for your inquiry. This PA1303 anti-S100 beta/S100B antibody is tested on rat brain tissue, tissue lysate, hela cell lysate, smmc cell lysate, jurkat cell lysate, colo320 cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-01-02
Question
Is a blocking peptide available for product anti-S100 beta/S100B antibody (PA1303)?
Verified Customer
Verified customer
Asked: 2018-04-09
Answer
We do provide the blocking peptide for product anti-S100 beta/S100B antibody (PA1303). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-04-09
Question
My team were content with the WB result of your anti-S100 beta/S100B antibody. However we have observed positive staining in skin cytoplasm. using this antibody. Is that expected? Could you tell me where is S100B supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-09-04
Answer
From literature, skin does express S100B. Generally S100B expresses in cytoplasm. Regarding which tissues have S100B expression, here are a few articles citing expression in various tissues:
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-09-04
Question
Is there a BSA free version of anti-S100 beta/S100B antibody PA1303 available?
K. Lewis
Verified customer
Asked: 2017-01-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-S100 beta/S100B antibody PA1303 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-01-23
Question
Does PA1303 anti-S100 beta/S100B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Miller
Verified customer
Asked: 2017-01-13
Answer
You can see on the product datasheet, PA1303 anti-S100 beta/S100B antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-01-13
