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Product Info Summary
| SKU: | M00213-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GFAP Rabbit Monoclonal Antibody
SKU/Catalog Number
M00213-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GFAP Rabbit Monoclonal Antibody catalog # M00213-2. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GFAP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00213-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ECO-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GFAP
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00213-2 is reactive to GFAP in Human, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
30 kDa
Calculated molecular weight
49880 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00213-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: human glioma tissue
ICC/IF: SNB19 cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of GFAP expression in Rat brain lysate.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human glioma, using GFAP Antibody .
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunofluorescent analysis of SNB19 cells, using GFAP Antibody.
Protein Target Info & Infographic
Gene/Protein Information For GFAP (Source: Uniprot.org, NCBI)
Gene Name
GFAP
Full Name
Glial fibrillary acidic protein
Weight
49880 MW
Superfamily
intermediate filament family
Alternative Names
Glial fibrillary acidic protein;GFAP;GFAP; GFAP ALXDRD glial fibrillary acidic protein glial fibrillary acidic protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GFAP, check out the GFAP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GFAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GFAP Rabbit Monoclonal Antibody (M00213-2)
Hello CJ!
M00213-2 has been cited in 43 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang LY,Jin QQ,Hölscher C,Li L.Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6-hydroxydopamine rat Parkinson model. Neural Regen Res 2021;16:1660-70
Species: Human,Rat
M00213-2 usage in article: APP:IF, SAMPLE:SN TISSUE, DILUTION:1:100
Ahmed S. Ahmed,JAK-1/STAT-3 pathway mediated role in aging cerebellar cortex degenerative changes of albino wistar rats, Translational Research in Anatomy, 2020,100089,ISSN 2214-854X, https://doi.org/10. 1016/j.tria.2020.100089.
Species: Rat
M00213-2 usage in article: APP:IHC, SAMPLE:CEREBELLAR CORTEX, DILUTION:1:20
Zhou Feng,Shengyan Liu,Qianwei Chen,Qiang Tan,Jishu Xian,Hua Feng,Zhi Chen,Gang Li,uPA alleviates kaolin-induced hydrocephalus by promoting the release and activation of hepatocyte growth factor in rats, Neuroscience Letters,Volume 731,2020,135011,ISSN 03
Species: Rat
Jiangong Wang,Bin Liu,Yong Xu et al. Inhibition of Histamine H3 receptor Attenuates Neuroinflammation and Cognitive Impairments in Alzheimer’s Disease via activating CREB Pathway,16 December 2020,PREPRINT (Version 1)available at Research Square [+https://
Species: Mouse
M00213-2 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:200
Luo Z,Wang J,Tang S,Zheng Y,Zhou X,Tian F,Xu Z.Dynamic-related protein 1 inhibitor eases epileptic seizures and can regulate equilibrative nucleoside transporter 1 expression.BMC Neurol.2020 Sep 22;20(1):353.doi:10.1186/s12883-020-01921-y.PMID:32962663;PM
Species: Rat
M00213-2 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:200
Wang X,Zhou W,Li X,Ren J,Ji G,Du J,Tian W,Liu Q,Hao A. Graphene oxide suppresses the growth and malignancy of glioblastoma stem cell-like spheroids via epigenetic mechanisms. J Transl Med.2020 May 14;18(1):200. doi:10.1186/s12967-020-02359-z.PMID:32410622
Species: Human
M00213-2 usage in article: APP:WB, SAMPLE:GSCS, DILUTION:1:1000
Li R,Wen Y,Wu B,He M,Zhang P,Zhang Q,Chen Y.MicroRNA-25-3p suppresses epileptiform discharges through inhibiting oxidative stress and apoptosis via targeting OXSR1 in neurons.Biochem Biophys Res Commun. 2020 Mar 19;523(4):859-866.doi:10.1016/j.bbrc.2020.
Species: Rat
M00213-2 usage in article: APP:IHC, SAMPLE:HIPPOCAMPUL NEURONS, DILUTION:NA
Deng Z,Wei Y,Yao Y,Gao S,Wang X.Let-7f promotes the differentiation of neural stem cells in rats.Am J Transl Res.2020 Sep 15;12(9):5752-5761.PMID:33042454;PMCID:PMC7540113.
Species: Rat
M00213-2 usage in article: APP:IF, SAMPLE:NSCS, DILUTION:1:100
Li X,Shi MQ,Chen C,Du JR.Phthalide derivative CD21 ameliorates ischemic brain injury in a mouse model of global cerebral ischemia: involvement of inhibition of NLRP3.Int Immunopharmacol.2020 Sep;86:106714.doi: 10.1016/j.intimp.2020.106714.Epub 2020 Jun 24
Species: Mouse
M00213-2 usage in article: APP:IF, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:100
Xue NY,Ge DY,Dong RJ,Kim HH,Ren XJ,Tu Y.Effect of electroacupuncture on glial fibrillary acidic protein and nerve growth factor in the hippocampus of rats with hyperlipidemia and middle cerebral artery thrombus. Neural Regen Res.2021 Jan;16(1):137-142.doi
Species: Rat
M00213-2 usage in article: APP:IHC, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:100
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-GFAP Rabbit Monoclonal Antibody
Question
We are currently using anti-GFAP Rabbit Monoclonal antibody M00213-2 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
The anti-GFAP Rabbit Monoclonal antibody (M00213-2) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-06
Question
Is this M00213-2 anti-GFAP Rabbit Monoclonal antibody reactive to the isotypes of GFAP?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
The immunogen of M00213-2 anti-GFAP Rabbit Monoclonal antibody is A synthesized peptide derived from human GFAP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-31
Question
We have been able to see staining in rat brain thalamus. Are there any suggestions? Is anti-GFAP Rabbit Monoclonal antibody supposed to stain brain thalamus positively?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
Based on literature brain thalamus does express GFAP. Based on Uniprot.org, GFAP is expressed in dorsal motor nucleus of vagus nerve, brain thalamus, brain, kidney, fetal brain cortex, fetal brain, blood, among other tissues. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2020-03-05
Question
Would anti-GFAP Rabbit Monoclonal antibody M00213-2 work for IF with fetal brain?
L. Miller
Verified customer
Asked: 2020-03-02
Answer
According to the expression profile of fetal brain, GFAP is highly expressed in fetal brain. So, it is likely that anti-GFAP Rabbit Monoclonal antibody M00213-2 will work for IF with fetal brain.
Boster Scientific Support
Answered: 2020-03-02
Question
I was wanting to use to test anti-GFAP Rabbit Monoclonal antibody M00213-2 on rat fetal brain for research purposes, then I may be interested in using anti-GFAP Rabbit Monoclonal antibody M00213-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The products we sell, including anti-GFAP Rabbit Monoclonal antibody M00213-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-04
Question
Can you help my question with product M00213-2, anti-GFAP Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00213-2 anti-GFAP Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-31
Question
Our lab used your anti-GFAP Rabbit Monoclonal antibody for IHC on brain thalamus in a previous project. I am using human, and We are going to use the antibody for IF next. I am looking for examining brain thalamus as well as kidney in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
I looked at the website and datasheets of our anti-GFAP Rabbit Monoclonal antibody and I see that M00213-2 has been validated on human in both IHC and IF. Thus M00213-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-23
Question
Will anti-GFAP Rabbit Monoclonal antibody M00213-2 work on horse IHC with fetal brain?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
Our lab technicians have not tested anti-GFAP Rabbit Monoclonal antibody M00213-2 on horse. You can run a BLAST between horse and the immunogen sequence of anti-GFAP Rabbit Monoclonal antibody M00213-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse fetal brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-27
Question
Is a blocking peptide available for product anti-GFAP Rabbit Monoclonal antibody (M00213-2)?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
We do provide the blocking peptide for product anti-GFAP Rabbit Monoclonal antibody (M00213-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-29
Question
Do you have a BSA free version of anti-GFAP Rabbit Monoclonal antibody M00213-2 available?
Verified Customer
Verified customer
Asked: 2019-01-24
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-GFAP Rabbit Monoclonal antibody M00213-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-24
Question
I was wanting to use your anti-GFAP Rabbit Monoclonal antibody for IF for rat fetal brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat fetal brain identification?
Verified Customer
Verified customer
Asked: 2018-11-22
Answer
It shows on the product datasheet, M00213-2 anti-GFAP Rabbit Monoclonal antibody has been validated for IF, IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fetal brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-22
Question
My colleagues were content with the WB result of your anti-GFAP Rabbit Monoclonal antibody. However we have seen positive staining in blood cytoplasm. using this antibody. Is that expected? Could you tell me where is GFAP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-07-09
Answer
From literature, blood does express GFAP. Generally GFAP expresses in cytoplasm. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2018-07-09
Question
I see that the anti-GFAP Rabbit Monoclonal antibody M00213-2 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-11-17
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-11-17
Question
Would M00213-2 anti-GFAP Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-17
Answer
As indicated on the product datasheet, M00213-2 anti-GFAP Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-17
Question
Here is the WB image, lot number and protocol we used for fetal brain using anti-GFAP Rabbit Monoclonal antibody M00213-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-07-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-06
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-GFAP Rabbit Monoclonal antibody M00213-2. Let me know if you need anything else.
B. Edwards
Verified customer
Asked: 2013-09-19
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-09-19
