Anti-Gelsolin Antibody (Monoclonal, GS-2C4)
|Ig Isotype||Mouse IgG1|
|Product Name||Anti-Gelsolin Antibody (Monoclonal, GS-2C4)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for Gelsolin, gelsolin (GSN) detection. Tested with WB, IHC-P, IHC-F in Human; rabbit. No cross reactivity with other proteins.|
|Cite This Product||Anti-Gelsolin Antibody (Monoclonal, GS-2C4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1044)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Human plasma gelsolin.|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, rabbit, By Heat
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, rabbit, -
Western blot, 0.5-1μg/ml, Human, rabbit
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
Images And Assay Conditions
Anti- Gelsolin antibody, MA1044, IHC(P)
IHC(P): Human Lung Cancer Tissue
Anti- Gelsolin antibody, MA1044, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Figure 3. Western blot analysis of Gelsolin using anti-Gelsolin antibody (MA1044).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of s
Protein Target Info (Source: Uniprot.org)
|Tissue Specificity||GSN: Phagocytic cells, platelets, fibroblasts,nonmuscle cells, smooth and skeletal muscle cells.|
|Alternative Names||Gelsolin;Actin-depolymerizing factor;ADF;Brevin;Gsn;|
|Subcellular Localization||Isoform 1: Secreted .|
|Molecular Weight||86068 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis (By similarity). .|
|Research Areas||Human, Rabbit
*You can search these to find other products in these research areas.
|Background||Gelsolin, a protein of leukocytes, platelets, and other cells, severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A gelsolin variant with 23 more N-terminal amino acids is a plasma component probably involved in the clearance of actin, the most abundant human protein, from the circulation. Gelsolin is located in 9q34. Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain.|
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Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to [email protected]