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Product Info Summary
| SKU: | EK1571 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat beta IG-H3/TGFBI ELISA Kit PicoKine®
View all Beta Ig-h3/TGFBI ELISA kits
SKU/Catalog Number
EK1571
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat beta IG-H3/TGFBI ELISA Kit PicoKine® (96 Tests). Quantitate Rat TGFBI in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat beta IG-H3/TGFBI ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1571)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: G24-G685
Sensitivity
<15 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1571 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1571 is reactive to TGFBI in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1571 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Beta Ig-h3/TGFBI
Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. It is induced by transforming growth factor-beta and acts to inhibit cell adhesion.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1571-CAP | Anti-Rat TGFBI Pre-coated 96-well strip microplate | 1 |
| EK1571-ST | Rat TGFBI Standard | 2 vials, 10 ng/tube |
| EK1571-DA | Rat TGFBI Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.010 | 0.031 | 0.064 | 0.137 | 0.376 | 0.922 | 1.352 | 1.879 |
Data Example Images
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Rat beta IG-H3/TGFBI PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Beta Ig-h3/TGFBI, Dilution ratio of 1:200, concentration in serum and plasma is 200-489 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat beta IG-H3/TGFBI ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 308 | 1355 | 5623 | 336 | 1296 | 5160 |
| Standard deviation | 20.63 | 73.17 | 314.88 | 24.19 | 84.24 | 309.6 |
| CV (%) | 6.7% | 5.4% | 5.6% | 7.2% | 6.5% | 6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Beta Ig-h3/TGFBI in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 308 | 274 | 298 | 308 | 297 | 13.89 | 4.6% |
| Sample 2 | 1355 | 1497 | 1581 | 1353 | 1446 | 97.15 | 6.7% |
| Sample 3 | 5623 | 6215 | 5716 | 5905 | 5864 | 226.3 | 3.8% |
Protein Target Info & Infographic
Gene/Protein Information For TGFBI (Source: Uniprot.Org, NCBI)
Gene Name
TGFBI
Full Name
Transforming growth factor-beta-induced protein ig-h3
Weight
74681 MW
Alternative Names
Transforming growth factor-beta-induced protein ig-h3;Beta ig-h3;Kerato-epithelin;RGD-containing collagen-associated protein;RGD-CAP;TGFBI;BIGH3; TGFBI BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, LCD1 transforming growth factor beta induced transforming growth factor-beta-induced protein ig-h3|RGD-CAP|RGD-containing collagen-associated protein|beta ig-h3|betaig-h3|kerato-epithelin|transforming growth factor beta-induced 68kDa|transforming growth factor, beta-induced, 68kD
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TGFBI, check out the TGFBI Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TGFBI: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat beta IG-H3/TGFBI ELISA Kit PicoKine® (EK1571)
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Customer Q&As
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8 Customer Q&As for Rat beta IG-H3/TGFBI ELISA Kit PicoKine®
Question
Q: which procedure should I follow in order to thaw whole blood sample for Beta Ig-h3 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-10-10
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Beta Ig-h3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-10-10
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Beta Ig-h3 using the EK1571 Rat Beta Ig-h3 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-06-25
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-06-25
Question
Q: if the enzyme conjugated Beta Ig-h3 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Beta Ig-h3 antigen?
K. Chen
Verified customer
Asked: 2019-07-23
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-07-23
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-02-19
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-02-19
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
R. Mangal
Verified customer
Asked: 2018-02-06
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-02-06
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-01-27
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-01-27
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2017-04-17
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-04-17
Question
Q: What is the optimal O.D. value for Beta Ig-h3 ELISA kit? I performed your Beta Ig-h3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Beta Ig-h3 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2017-02-07
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Beta Ig-h3 ELISA kit to have sensitivity of 15pg/ml, that means the minimum amount of Beta Ig-h3 that can be declared/interpreted as positive by the above standard is 15pg/ml.
Boster Scientific Support
Answered: 2017-02-07
