Data

Array ( [0] => store_id [1] => entity_id [2] => attribute_set_id [3] => type_id [4] => sku [5] => has_options [6] => required_options [7] => created_at [8] => updated_at [9] => mst_search_weight [10] => status [11] => name [12] => cj_search_index [13] => publication_count [14] => qty_in_warehouses [15] => is_recurring [16] => visibility [17] => tax_class_id [18] => conjugate [19] => featured [20] => featured_image [21] => fb_product [22] => custom_product_page [23] => free_shipping [24] => template [25] => host [26] => quantity_and_stock_status [27] => googleshopping_exclude [28] => yoast_keyword_score [29] => yoast_content_score [30] => price [31] => weight [32] => meta_title [33] => meta_description [34] => image [35] => small_image [36] => thumbnail [37] => gallery [38] => url_key [39] => url_path [40] => options_container [41] => image_label [42] => small_image_label [43] => thumbnail_label [44] => msrp_display_actual_price_type [45] => gift_message_available [46] => size [47] => protein_name [48] => gene_full_name [49] => uniprot_id [50] => reconstitution [51] => sensitivity [52] => applications [53] => reactivity [54] => form [55] => concentration [56] => cross_reactivity [57] => clone_number [58] => predicted_reactivity [59] => source_company [60] => isotype [61] => molecular_weight [62] => sample_size_available [63] => related_product_skus [64] => product_category [65] => research_category [66] => clonality [67] => swatch_image [68] => rating_value [69] => review_count [70] => phospho_site [71] => mp_exclude_sitemap [72] => yoast_facebook_image [73] => yoast_twitter_image [74] => cj_related_products [75] => description [76] => short_description [77] => meta_keyword [78] => contents [79] => background [80] => gene_name [81] => synonyms [82] => kit_components [83] => application_details [84] => subcellular_localization [85] => tissue_specificity [86] => protein_function [87] => application_notes [88] => competitor_equivalent_skus [89] => immunogen [90] => purification [91] => storage [92] => reproducibility [93] => custom_attribute_1 [94] => custom_attribute_2 [95] => custom_attribute_3 [96] => options [97] => media_gallery [98] => extension_attributes [99] => tier_price [100] => tier_price_changed [101] => category_ids [102] => is_salable [103] => website_ids [104] => request_path [105] => rating_summary [106] => _cache_instance_store_filter [107] => media_gallery_images )
Key=store_id
Key=entity_id, value=120104
Key=attribute_set_id, value=4
Key=type_id, value=simple
Key=sku, value=M00227-7
Key=has_options, value=1
Key=required_options, value=1
Key=created_at, value=2021-09-23 06:52:58
Key=updated_at, value=2024-10-30 05:01:52
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Anti-GAPDH Mouse Monoclonal Antibody
Key=cj_search_index
Key=publication_count, value=0
Key=qty_in_warehouses
Key=is_recurring, value=No
Key=visibility, value=Catalog, Search
Key=tax_class_id, value=Taxable Goods
Key=conjugate
Key=featured, value=No
Key=featured_image, value=Yes
Key=fb_product, value=
Key=custom_product_page, value=No
Key=free_shipping, value=No
Key=template, value=antibodies
Key=host, value=Mouse
Key=quantity_and_stock_status
Key=googleshopping_exclude, value=No
Key=yoast_keyword_score, value=-1369
Key=yoast_content_score, value=60
Key=price, value=370.0000
Key=weight, value=1.0000
Key=meta_title, value=Anti-GAPDH Mouse Monoclonal Antibody
Key=meta_description, value=Boster Bio Anti-GAPDH Mouse Monoclonal Antibody catalog # M00227-7. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key=image, value=/m/0/m00227-1-gapdh-primary-antibodies-wb-testing-1_1_1.jpg
Key=small_image, value=/m/0/m00227-1-gapdh-primary-antibodies-wb-testing-1_1_1.jpg
Key=thumbnail, value=/m/0/m00227-1-gapdh-primary-antibodies-wb-testing-1_1_1.jpg
Key=gallery, value=/A00237_1.jpg:/A00237_2.jpg:/A00237_3.jpg
Key=url_key, value=anti-gapdh-mouse-monoclonal-antibody-m00227-7-boster
Key=url_path, value=anti-gapdh-rabbit-monoclonal-antibody-m00227-1-boster
Key=options_container, value=Product Info Column
Key=image_label, value=Western blot analysis of GAPDH expression in (1) HeLa cell lysate; (2) MDBK cell lysate; (3) COS-1 cell lysate; (4) MDCK cell lysate with GAPDH Antibody.
Key=small_image_label, value=Western blot analysis of GAPDH expression in (1) HeLa cell lysate; (2) MDBK cell lysate; (3) COS-1 cell lysate; (4) MDCK cell lysate with GAPDH Antibody.
Key=thumbnail_label, value=Western blot analysis of GAPDH expression in (1) HeLa cell lysate; (2) MDBK cell lysate; (3) COS-1 cell lysate; (4) MDCK cell lysate with GAPDH Antibody.
Key=msrp_display_actual_price_type, value=Use config
Key=gift_message_available, value=No
Key=size, value=100 μl/vial
Key=protein_name, value=P2X purinoceptor 1
Key=gene_full_name, value=Glyceraldehyde-3-phosphate dehydrogenase
Key=uniprot_id, value=P04406
Key=reconstitution, value=Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Key=sensitivity
Key=applications, value=Flow Cytometry, IP, IF, IHC, ICC, WB
Key=reactivity, value=Human, Mouse, Rat
Key=form, value=Liquid
Key=concentration, value=Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Key=cross_reactivity
Key=clone_number, value=17G14
Key=predicted_reactivity
Key=source_company, value=Boster Amways (order through China) AB0038, Cost 100ul 580RMB, 30ul 210RMB
Key=isotype, value=IgG
Key=molecular_weight, value=36053 MW
Key=sample_size_available, value=30ul for $99, contact us for details
Key=related_product_skus, value=A00227,A00227-1,H00227,M00227,PB9107,PROTP04406,PROTP04406-1,PROTP16858,PROTP16858-1
Key=product_category, value=Primary Antibodies, Monoclonal Antibodies
Key=research_category, value=Alzheimer's Disease, Cancer, Cancer Metabolism, Carbohydrate Metabolism, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Carbohydrates, Neurodegenerative Disease, Neurology Process, Neuroscience, Pathways and Processes, Signal Transduction
Key=clonality, value=Monoclonal
Key=swatch_image, value=/antibody/ek0660-2-ELISA-mouse-axl-picokine-elisa-kit.jpg
Key=rating_value, value=100
Key=review_count, value=24
Key=phospho_site
Key=mp_exclude_sitemap, value=No
Key=yoast_facebook_image, value=no_selection
Key=yoast_twitter_image, value=no_selection
Key=cj_related_products, value=Loading Controls:M01263^Nuclear Receptors, Coactivators and Corepressors:A00227|A00227-1|M00227-6|M00227-4-1|PROTO75496
Key=description, value=Boster Bio Anti-GAPDH Mouse Monoclonal Antibody catalog # M00227-7. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key=short_description, value=Boster Bio Anti-GAPDH Mouse Monoclonal Antibody catalog # M00227-7. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key=meta_keyword, value=Anti-GAPDH Mouse Monoclonal Antibody
Key=contents, value=Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Key=background
Key=gene_name, value=GAPDH
Key=synonyms, value=Glyceraldehyde-3-phosphate dehydrogenase;GAPDH;1.2.1.12;Peptidyl-cysteine S-nitrosylase GAPDH;2.6.99.-;GAPDH;GAPD;CDABP0047, OK/SW-cl.12;
Key=kit_components, value=-
Key=application_details, value=WB 1:5000-1:20000
IHC 1:100-1:500
ICC/IF 1:100-1:250
IP 1:50
FC 1:50
Key=subcellular_localization, value= Cytoplasm, cytosol . Nucleus . Cytoplasm, perinuclear region . Membrane . Cytoplasm, cytoskeleton . Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. .
Key=tissue_specificity, value= Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle. .
Key=protein_function, value= Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. .
Key=application_notes, value=Specific for endogenous levels of the ~100 kDa GluR1 protein phosphorylated at Ser845. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Key=competitor_equivalent_skus, value=sc 165863|sc 165862|sc 292072|sc 165861|sc 101493|sc 390197|sc 390231|sc 390211
Key=immunogen, value=A synthesized peptide derived from human GAPDH Mouse Monoclonal
Key=purification, value=Affinity-chromatography
Key=storage, value=Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Key=reproducibility, value=-
Key=custom_attribute_1, value=58 kDa
Key=custom_attribute_2, value=WB: Hela cell, Jurkat cell, Mouse kidney, Mouse spleen, RAW 2647 cell, Rat brain
IHC: human colon cancer tissue
ICC/IF: Hela cell
Key=custom_attribute_3, value=BM3876
Key=options
Key=media_gallery
Key=extension_attributes
Key=tier_price
Key=tier_price_changed
Key=category_ids
Key=is_salable
Key=website_ids
Key=request_path
Key=rating_summary
Key=_cache_instance_store_filter
Key=media_gallery_images

Anti-GAPDH Mouse Monoclonal Antibody

GAPDH antibody

Boster Bio Anti-GAPDH Mouse Monoclonal Antibody catalog # M00227-7. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00227-7
Size: 100 μl/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

Customers Who Bought This Also Bought

Product Name

Anti-GAPDH Mouse Monoclonal Antibody

View all GAPDH Antibodies

SKU/Catalog Number

M00227-7

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-GAPDH Mouse Monoclonal Antibody catalog # M00227-7. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GAPDH Mouse Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00227-7)

Host

Mouse

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

17G14

Isotype

IgG

Immunogen

A synthesized peptide derived from human GAPDH Mouse Monoclonal

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00227-7 is reactive to GAPDH in Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

58 kDa

Calculated molecular weight

36053 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M00227-7 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:5000-1:20000
IHC 1:100-1:500
ICC/IF 1:100-1:250
IP 1:50
FC 1:50

Positive Control

WB: Hela cell, Jurkat cell, Mouse kidney, Mouse spleen, RAW 2647 cell, Rat brain
IHC: human colon cancer tissue
ICC/IF: Hela cell

Validation Images & Assay Conditions

Gene/Protein Information For GAPDH (Source: Uniprot.org, NCBI)

Gene Name

GAPDH

Full Name

Glyceraldehyde-3-phosphate dehydrogenase

Weight

36053 MW

Superfamily

glyceraldehyde-3-phosphate dehydrogenase family

Alternative Names

Glyceraldehyde-3-phosphate dehydrogenase;GAPDH;1.2.1.12;Peptidyl-cysteine S-nitrosylase GAPDH;2.6.99.-;GAPDH;GAPD;CDABP0047, OK/SW-cl.12; GAPDH G3PD, GAPD, HEL-S-162eP glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase|OCAS, p38 component|Oct1 coactivator in S phase, 38 Kd component|aging-associated gene 9 protein|epididymis secretory sperm binding protein Li 162eP|peptidyl-cysteine S-nitrosylase GAPDH

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GAPDH, check out the GAPDH Infographic

GAPDH infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GAPDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for M00227-7

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-GAPDH Mouse Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-GAPDH Mouse Monoclonal Antibody

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for Anti-GAPDH Mouse Monoclonal Antibody

Order DetailsPrice
M00227-7

100μL

$370
M00227-7-30ul

30μL sample

$99
M00227-7-carrier-free-1ml

Carrier Free

$2590

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
M00227-7
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.