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Key=store_id Key=entity_id, value=120104 Key=attribute_set_id, value=4 Key=type_id, value=simple Key=sku, value=M00227-7 Key=has_options, value=1 Key=required_options, value=1 Key=created_at, value=2021-09-23 06:52:58 Key=updated_at, value=2024-10-30 05:01:52 Key=mst_search_weight, value=0 Key=status, value=Enabled Key=name, value=Anti-GAPDH Mouse Monoclonal Antibody Key=cj_search_index Key=publication_count, value=0 Key=qty_in_warehouses Key=is_recurring, value=No Key=visibility, value=Catalog, Search Key=tax_class_id, value=Taxable Goods Key=conjugate Key=featured, value=No Key=featured_image, value=Yes Key=fb_product, value= Key=custom_product_page, value=No Key=free_shipping, value=No Key=template, value=antibodies Key=host, value=Mouse Key=quantity_and_stock_status Key=googleshopping_exclude, value=No Key=yoast_keyword_score, value=-1369 Key=yoast_content_score, value=60 Key=price, value=370.0000 Key=weight, value=1.0000 Key=meta_title, value=Anti-GAPDH Mouse Monoclonal Antibody Key=meta_description, value=Boster Bio Anti-GAPDH Mouse Monoclonal Antibody catalog # M00227-7. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Key=image, value=/m/0/m00227-1-gapdh-primary-antibodies-wb-testing-1_1_1.jpg Key=small_image, value=/m/0/m00227-1-gapdh-primary-antibodies-wb-testing-1_1_1.jpg Key=thumbnail, value=/m/0/m00227-1-gapdh-primary-antibodies-wb-testing-1_1_1.jpg Key=gallery, value=/A00237_1.jpg:/A00237_2.jpg:/A00237_3.jpg Key=url_key, value=anti-gapdh-mouse-monoclonal-antibody-m00227-7-boster Key=url_path, value=anti-gapdh-rabbit-monoclonal-antibody-m00227-1-boster Key=options_container, value=Product Info Column Key=image_label, value=Western blot analysis of GAPDH expression in (1) HeLa cell lysate; (2) MDBK cell lysate; (3) COS-1 cell lysate; (4) MDCK cell lysate with GAPDH Antibody. Key=small_image_label, value=Western blot analysis of GAPDH expression in (1) HeLa cell lysate; (2) MDBK cell lysate; (3) COS-1 cell lysate; (4) MDCK cell lysate with GAPDH Antibody. Key=thumbnail_label, value=Western blot analysis of GAPDH expression in (1) HeLa cell lysate; (2) MDBK cell lysate; (3) COS-1 cell lysate; (4) MDCK cell lysate with GAPDH Antibody. Key=msrp_display_actual_price_type, value=Use config Key=gift_message_available, value=No Key=size, value=100 μl/vial Key=protein_name, value=P2X purinoceptor 1 Key=gene_full_name, value=Glyceraldehyde-3-phosphate dehydrogenase Key=uniprot_id, value=P04406 Key=reconstitution, value=Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL Key=sensitivity Key=applications, value=Flow Cytometry, IP, IF, IHC, ICC, WB Key=reactivity, value=Human, Mouse, Rat Key=form, value=Liquid Key=concentration, value=Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. Key=cross_reactivity Key=clone_number, value=17G14 Key=predicted_reactivity Key=source_company, value=Boster Amways (order through China) AB0038, Cost 100ul 580RMB, 30ul 210RMB Key=isotype, value=IgG Key=molecular_weight, value=36053 MW Key=sample_size_available, value=30ul for $99, contact us for details Key=related_product_skus, value=A00227,A00227-1,H00227,M00227,PB9107,PROTP04406,PROTP04406-1,PROTP16858,PROTP16858-1 Key=product_category, value=Primary Antibodies, Monoclonal Antibodies Key=research_category, value=Alzheimer's Disease, Cancer, Cancer Metabolism, Carbohydrate Metabolism, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Carbohydrates, Neurodegenerative Disease, Neurology Process, Neuroscience, Pathways and Processes, Signal Transduction Key=clonality, value=Monoclonal Key=swatch_image, value=/antibody/ek0660-2-ELISA-mouse-axl-picokine-elisa-kit.jpg Key=rating_value, value=100 Key=review_count, value=24 Key=phospho_site Key=mp_exclude_sitemap, value=No Key=yoast_facebook_image, value=no_selection Key=yoast_twitter_image, value=no_selection Key=cj_related_products, value=Loading Controls:M01263^Nuclear Receptors, Coactivators and Corepressors:A00227|A00227-1|M00227-6|M00227-4-1|PROTO75496 Key=description, value=Boster Bio Anti-GAPDH Mouse Monoclonal Antibody catalog # M00227-7. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Key=short_description, value=Boster Bio Anti-GAPDH Mouse Monoclonal Antibody catalog # M00227-7. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Key=meta_keyword, value=Anti-GAPDH Mouse Monoclonal Antibody Key=contents, value=Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. Key=background Key=gene_name, value=GAPDH Key=synonyms, value=Glyceraldehyde-3-phosphate dehydrogenase;GAPDH;1.2.1.12;Peptidyl-cysteine S-nitrosylase GAPDH;2.6.99.-;GAPDH;GAPD;CDABP0047, OK/SW-cl.12; Key=kit_components, value=- Key=application_details, value=WB 1:5000-1:20000 IHC 1:100-1:500 ICC/IF 1:100-1:250 IP 1:50 FC 1:50 Key=subcellular_localization, value= Cytoplasm, cytosol . Nucleus . Cytoplasm, perinuclear region . Membrane . Cytoplasm, cytoskeleton . Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. . Key=tissue_specificity, value= Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle. . Key=protein_function, value= Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. . Key=application_notes, value=Specific for endogenous levels of the ~100 kDa GluR1 protein phosphorylated at Ser845. Immunolabeling is completely eliminated by treatment with λ-phosphatase. Key=competitor_equivalent_skus, value=sc 165863|sc 165862|sc 292072|sc 165861|sc 101493|sc 390197|sc 390231|sc 390211 Key=immunogen, value=A synthesized peptide derived from human GAPDH Mouse Monoclonal Key=purification, value=Affinity-chromatography Key=storage, value=Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Key=reproducibility, value=- Key=custom_attribute_1, value=58 kDa Key=custom_attribute_2, value=WB: Hela cell, Jurkat cell, Mouse kidney, Mouse spleen, RAW 2647 cell, Rat brain IHC: human colon cancer tissue ICC/IF: Hela cell Key=custom_attribute_3, value=BM3876 Key=options Key=media_gallery Key=extension_attributes Key=tier_price Key=tier_price_changed Key=category_ids Key=is_salable Key=website_ids Key=request_path Key=rating_summary Key=_cache_instance_store_filter Key=media_gallery_images
Anti-GAPDH Mouse Monoclonal Antibody
GAPDH antibody
Boster Bio Anti-GAPDH Mouse Monoclonal Antibody catalog # M00227-7. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Boster Bio Anti-GAPDH Mouse Monoclonal Antibody catalog # M00227-7. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GAPDH Mouse Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00227-7)
Host
Mouse
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
17G14
Isotype
IgG
Immunogen
A synthesized peptide derived from human GAPDH Mouse Monoclonal
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00227-7 is reactive to GAPDH in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
58 kDa
Calculated molecular weight
36053 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
M00227-7 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:20000 IHC 1:100-1:500 ICC/IF 1:100-1:250 IP 1:50 FC 1:50
Positive Control
WB: Hela cell, Jurkat cell, Mouse kidney, Mouse spleen, RAW 2647 cell, Rat brain IHC: human colon cancer tissue ICC/IF: Hela cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of GAPDH expression in (1) Hela cell lysate; (2)Jurkat cell lysate; (3)Mouse kidney lysate; (4) Mouse spleen lysate; (5) RAW 264.7 cell lysate; (6) Rat brain lysate with GAPDH Mouse Monoclonal Antibody.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human colon cancer, using GAPDH Mouse Monoclonal Antibody.
Click image to see more details
Immunofluorescent analysis of Hela cells, using GAPDH Mouse Monoclonal Antibody.
Gene/Protein Information For GAPDH (Source: Uniprot.org, NCBI)
Gene Name
GAPDH
Full Name
Glyceraldehyde-3-phosphate dehydrogenase
Weight
36053 MW
Superfamily
glyceraldehyde-3-phosphate dehydrogenase family
Alternative Names
Glyceraldehyde-3-phosphate dehydrogenase;GAPDH;1.2.1.12;Peptidyl-cysteine S-nitrosylase GAPDH;2.6.99.-;GAPDH;GAPD;CDABP0047, OK/SW-cl.12; GAPDH G3PD, GAPD, HEL-S-162eP glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase|OCAS, p38 component|Oct1 coactivator in S phase, 38 Kd component|aging-associated gene 9 protein|epididymis secretory sperm binding protein Li 162eP|peptidyl-cysteine S-nitrosylase GAPDH
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on GAPDH, check out the GAPDH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GAPDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.