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Product Info Summary
| SKU: | H00227 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated
SKU/Catalog Number
H00227
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated catalog # H00227. Tested in WB application. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # H00227)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
A-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GAPDH (HRP conjugated)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
H00227 is reactive to GAPDH in Human, Monkey, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
68 kDa
Calculated molecular weight
36053 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
H00227 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:20000
Positive Control
WB: Jurkat cell, A375 cell, Human hippocampus, Human fetal liver, COS-1 cell, Raw2647 cell, Mouse kidney, PC-12 cell, Rat brain
Validation Images & Assay Conditions
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Western blot analysis of GAPDH expression in (1) Jurkat cell lysate; (2) A375 cell lysate; (3) Human hippocampus lysate; (4) Human fetal liver lysate; (5) COS-1 cell lysate; (6) Raw264.7 cell lysate; (7) Mouse kidney lysate; (8) PC-12 cell lysate; (9) Rat brain lysate
Protein Target Info & Infographic
Gene/Protein Information For GAPDH (Source: Uniprot.org, NCBI)
Gene Name
GAPDH
Full Name
Glyceraldehyde-3-phosphate dehydrogenase
Weight
36053 MW
Superfamily
glyceraldehyde-3-phosphate dehydrogenase family
Alternative Names
Glyceraldehyde-3-phosphate dehydrogenase;GAPDH;1.2.1.12;Peptidyl-cysteine S-nitrosylase GAPDH;2.6.99.-;GAPDH;GAPD;CDABP0047, OK/SW-cl.12; GAPDH G3PD, GAPD, HEL-S-162eP glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase|OCAS, p38 component|Oct1 coactivator in S phase, 38 Kd component|aging-associated gene 9 protein|epididymis secretory sperm binding protein Li 162eP|peptidyl-cysteine S-nitrosylase GAPDH
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GAPDH, check out the GAPDH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GAPDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated (H00227)
Hello CJ!
H00227 has been cited in 161 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang H,Chen H,Jiang Z,Lin Y,Wang X,Xiang J,Peng J.Integrin subunit alpha V promotes growth, migration, and invasion of gastric cancer cells.Pathol Res Pract.2019 Sep;215(9):152531.doi:10.1016/j.prp.2019.152531.Epub 2019 Jul 10.PMID:31320250.
Species: Human
H00227 usage in article: APP:WB, SAMPLE:GC CELL, DILUTION:1:500
Huang W,Guo L,Zhao M,Zhang D,Xu H,Nie Q.The Inhibition on MDFIC and PI3K/AKT Pathway Caused by miR-146b-3p Triggers Suppression of Myoblast Proliferation and Differentiation and Promotion of Apoptosis.Cells.2019 Jun 29;8(7):656.doi:10.3390/cells8070656.PMID:31261950; PMCID:PMC6678156.
Species: Chicken
H00227 usage in article: APP:WB, SAMPLE:QM-7 CELL, DILUTION:1:2000
Xiang W,Jiang T,Hao X, Wang R,Yao X,Sun K,Guo F,Xu T.Primary cilia and autophagy interaction is involved in mechanical stress mediated cartilage development via ERK/mTOR axis.Life Sci.2019 Feb 1;218:308-313.doi:10.1016/j. lfs.2019.01.001.Epub 2019 Jan 3.PMID:30610869.
Species: Rat
H00227 usage in article: APP:WB, SAMPLE:CHONDROCYTES, DILUTION:1:400
Xie R,Liu J,Yu X,Li C,Wang Y,Yang W,Hu J,Liu P,Sui H,Liang P,Huang X,Wang L,Bai Y,Xue Y,Zhu J,Fang T.ANXA2 Silencing Inhibits Proliferation, Invasion, and Migration in Gastric Cancer Cells.J Oncol.2019 May 2;2019:4035460.doi:10.1155/2019/4035460.PMID:31186633;PMCID:PMC6521490.
Species: Human
H00227 usage in article: APP:WB, SAMPLE:SGC-7901 CELL,MKN-45 CELL,BGC-823 CELL AND AGS CELL, DILUTION:NA
Sheng X,Li Y,Li Y,Liu W,Lu Z,Zhan J,Xu M,Chen L,Luo X,Cai G,Zhang S.PLOD2 contributes to drug resistance in laryngeal cancer by promoting cancer stem cell-like characteristics.BMC Cancer.2019 Aug 27;19(1):840.doi:10.1186/s12885-019-6029-y.PMID:31455288; PMCID:PMC6712771.
Species: Human,Mouse
H00227 usage in article: APP:WB, SAMPLE:FADU CELL,HEP2 CELL AND LSCC TISSUE, DILUTION:1:3000
Nina Yin,Man Long,Zhigang Wang et al.Electroacupuncture Pretreatment Attenuates Cerebral Ischemia-Reperfusion Injury through Transient Receptor Potential Vanilloid 1-Mediated Anti-Apoptosis via Inhibiting NF-kB Signaling Pathway,30 June 2021,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-657177/v1]
Species: Rat
H00227 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS TISSUE, DILUTION:NA
Jia Han,Wei Hou,Bi-qing Cai,Fan Zhang,Jian-cai Tang,"12-Epi-Napelline Inhibits Leukemia Cell Proliferation via the PI3K/AKT Signaling Pathway In Vitro and In Vivo",Evidence-Based Complementary and Alternative Medicine,vol.2021,Article ID 6687519,12 pages,2021.https://doi.org/10.1155/2021/6687519
Species: Human,Mouse
H00227 usage in article: APP:WB, SAMPLE:K-562 CELL, DILUTION:NA
Enoch Appiah Adu-Gyamfi,Jones Lamptey,Xue-Mei Chen,Fang-Fang Li,Cong Li,Ling-Ling Ruan,Xue-Niu Yang,Tai-Hang Liu,Ying-Xiong Wang,Yu-Bin Ding,Iodothyronine deiodinase 2 (DiO2) regulates trophoblast cell line cycle, invasion and apoptosis; and its downregulation is associated with early recurrent miscarriage,Placenta,Volume 111,2021,Pages 54-68,ISSN 0143-4004,https://doi.org/10.1016/j.placenta.2021.06.004.
Species: Human
H00227 usage in article: APP:WB, SAMPLE:HTR-8/SVNEO CELL, DILUTION:NA
Tibatan MA,Önay Uçar E.Long non-coding RNA NKILA regulates expression of HSP90α, NF-κB and β-catenin proteins in the MCF-7 breast cancer cell line.Mol Biol Rep.2021 Jun 14.doi:10.1007/s11033-021-06482-y.Epub ahead of print.PMID:34125329.
Species: Human
H00227 usage in article: APP:WB, SAMPLE:MCF-7 CELL, DILUTION:NA
Xiaoming Zhang,Ying-Hong Tao,Xiu-Ling Zhou et al.The Role of Carbonic Anhydrase Ⅲ and Autophagy in Type 2 Diabetes with Cardio-Cerebrovascular Disease,08 June 2021,PREPRINT(Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-495613/v1]
Species: Mouse
H00227 usage in article: APP:WB, SAMPLE:CORTEX AND MYOCARDIUM, DILUTION:1:2000
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Customer Q&As
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4 Customer Q&As for Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated
Question
We have seen staining in mouse lung. Any tips? Is anti-GAPDH Rabbit Monoclonal antibody, HRP Conjugated supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
From literature lung does express GAPDH. From Uniprot.org, GAPDH is expressed in smooth muscle tissue, liver, lung, placenta, astrocytoma, leukemia, colon adenocarcinoma, eye, kidney, lung, lymph placenta, muscle, platelet, prostatic carcinoma, brain, cajal-retzius cell fetal brain cortex, heart, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have GAPDH expression, here are a few articles citing expression in various tissues:
Astrocytoma, Pubmed ID: 10944468
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 7498159
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 2987855, 24275569
Lung, Pubmed ID: 3664468
Lymphoblast, Pubmed ID: 14654843
Muscle, Pubmed ID: 1193541, 11724794
Placenta, Pubmed ID: 1924305
Boster Scientific Support
Answered: 2020-04-28
Question
My team were satisfied with the WB result of your anti-GAPDH Rabbit Monoclonal antibody, HRP Conjugated. However we have observed positive staining in lymphoblast cytosol using this antibody. Is that expected? Could you tell me where is GAPDH supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-06-13
Answer
From literature, lymphoblast does express GAPDH. Generally GAPDH expresses in cytoplasm, cytosol. Regarding which tissues have GAPDH expression, here are a few articles citing expression in various tissues:
Astrocytoma, Pubmed ID: 10944468
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 7498159
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 2987855, 24275569
Lung, Pubmed ID: 3664468
Lymphoblast, Pubmed ID: 14654843
Muscle, Pubmed ID: 1193541, 11724794
Placenta, Pubmed ID: 1924305
Boster Scientific Support
Answered: 2018-06-13
Question
We are currently using anti-GAPDH Rabbit Monoclonal antibody, HRP Conjugated H00227 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
The anti-GAPDH Rabbit Monoclonal antibody, HRP Conjugated (H00227) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-19
Question
We need using your anti-GAPDH Rabbit Monoclonal antibody, HRP Conjugated for killing of cells of other organism studies. Has this antibody been tested with western blotting on mouse kidney? We would like to see some validation images before ordering.
R. Huang
Verified customer
Asked: 2016-01-19
Answer
I appreciate your inquiry. This H00227 anti-GAPDH Rabbit Monoclonal antibody, HRP Conjugated is tested on jurkat cell lysate, mouse kidney. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-01-19
