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Product Info Summary
| SKU: | M00621-4 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Galectin 3 Rabbit Monoclonal Antibody
View all Galectin-3 Antibodies
SKU/Catalog Number
M00621-4
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Galectin 3 Rabbit Monoclonal Antibody catalog # M00621-4. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Galectin 3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00621-4)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
28L32
Isotype
IgG
Immunogen
A synthesized peptide derived from human Galectin 3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00621-4 is reactive to LGALS3 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
29 kDa
Calculated molecular weight
26152 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00621-4 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: human A431 whole cell, human SIHA whole cell, human RT4 whole cell, human MCF-7 whole cell, rat PC-12 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Galectin 3 using anti-Galectin 3 antibody (M00621-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human SIHA whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 3 antigen affinity purified monoclonal antibody (Catalog # M00621-4) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 3 at approximately 29 kDa. The expected band size for Galectin 3 is at 26 kDa.
Protein Target Info & Infographic
Gene/Protein Information For LGALS3 (Source: Uniprot.org, NCBI)
Gene Name
LGALS3
Full Name
Galectin-3
Weight
26152 MW
Alternative Names
Galectin-3;Gal-3;35 kDa lectin;Carbohydrate-binding protein 35;CBP 35;Galactose-specific lectin 3;Galactoside-binding protein;GALBP;IgE-binding protein;L-31;Laminin-binding protein;Lectin L-29;Mac-2 antigen;LGALS3;MAC2; LGALS3 CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2 galectin 3 galectin-3|35 kDa lectin|IgE-binding protein|MAC-2 |advanced glycation end-product receptor 3|carbohydrate-binding protein 35|epididymis secretory sperm binding protein|galactose-specific lectin 3|laminin-binding protein|lectin L-29|lectin, galactoside-binding, soluble, 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LGALS3, check out the LGALS3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LGALS3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Galectin 3 Rabbit Monoclonal Antibody (M00621-4)
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