Mouse IGFBP-1 ELISA Kit PicoKine®

Product Name

Mouse IGFBP-1 ELISA Kit PicoKine®

View all IGFBP-1 ELISA kits

SKU/Catalog Number

EK0383

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse IGFBP-1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Igfbp1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse IGFBP-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0383)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: A26-N272

Sensitivity

<3 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

See datasheet of EK0383 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0383 is reactive to Igfbp1 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0383 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IGFBP-1

IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7. The standard product used in this kit is recombinant mouse IGFBP-1, consisting of 247 amino acids with the molecular mass of 27KDa.

Kit Components

Catalog Number	Description	Quantity
EK0383-CAP	Anti-Mouse Igfbp1 Pre-coated 96-well strip microplate	1
EK0383-ST	Mouse Igfbp1 Standard	2 vials, 10 ng/tube
EK0383-DA	Mouse Igfbp1 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.128	0.188	0.240	0.331	0.581	0.941	1.498	1.909

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IGFBP-1, Dilution ratio of 1:10, concentration in serum and plasma is 2737-4530 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse IGFBP-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IGFBP-1 in ELISA kits from four different production batches/lots.

Gene/Protein Information For Igfbp1 (Source: Uniprot.Org, NCBI)

Alternative Names

Insulin-like growth factor-binding protein 1;IBP-1;IGF-binding protein 1;IGFBP-1;Igfbp1;Igfbp-1; IGFBP1 AFBP, IBP1, IGF-BP25, PP12, hIGFBP-1 insulin like growth factor binding protein 1 insulin-like growth factor-binding protein 1|IBP-1|IGF-binding protein 1|IGFBP-1|alpha-pregnancy-associated endometrial globulin|amniotic fluid binding protein|binding protein-25|binding protein-26|binding protein-28|growth hormone independent-binding protein|placental protein 12

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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