Click image to see more details
Product Info Summary
| SKU: | M00470-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Galectin 1 LGALS1 Monoclonal Antibody
View all Galectin-1 Antibodies
SKU/Catalog Number
M00470-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Galectin 1 LGALS1 Monoclonal Antibody catalog # M00470-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Galectin 1 LGALS1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00470-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADGB-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Galectin 1 May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00470-1 is reactive to LGALS1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
24 kDa, 29 kDa
Calculated molecular weight
114900 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00470-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Positive Control
WB: K562 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Galectin 1 expression in K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For LGALS1 (Source: Uniprot.org, NCBI)
Gene Name
LGALS1
Full Name
Galectin-1
Weight
114900 MW
Alternative Names
Integrin alpha-4;CD49 antigen-like family member D;Integrin alpha-IV;VLA-4 subunit alpha;CD49d;ITGA4;CD49D; LGALS1 GAL1, GBP galectin 1 galectin-1|14 kDa laminin-binding protein|14 kDa lectin|HBL|HLBP14|HPL|S-Lac lectin 1|beta-galactoside-binding lectin L-14-I|beta-galactoside-binding protein 14kDa|epididymis secretory sperm binding protein|gal-1|galaptin|lactose-binding lectin 1|lectin, galactoside-binding, soluble, 1|putative MAPK-activating protein PM12
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LGALS1, check out the LGALS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LGALS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Galectin 1 LGALS1 Monoclonal Antibody (M00470-1)
Hello CJ!
M00470-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ye Z,Huang S,Zhang Y,Mei X,Zheng H,Li M,Chen J,Lu F.Galectins, Eosinophiles, and Macrophages May Contribute to Schistosoma japonicum Egg-Induced Immunopathology in a Mouse Model.Front Immunol.2020 Mar 13;11:146.doi:10.3389/fimmu.2020.00146.PMID:32231658;
Species: Mouse
M00470-1 usage in article: APP:IHC, SAMPLE:LIVER,SPLEEN AND INTESTINE TISSUE, DILUTION:1:500
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Galectin 1 LGALS1 Monoclonal Antibody
Question
I see that the anti-Galectin 1 Monoclonal antibody M00470-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-01
Question
My boss were content with the WB result of your anti-Galectin 1 Monoclonal antibody. However we have observed positive staining in adipose tissue extracellular using this antibody. Is that expected? Could you tell me where is LGALS1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
According to literature, adipose tissue does express LGALS1. Generally LGALS1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have LGALS1 expression, here are a few articles citing expression in various tissues:
Hepatoma, Pubmed ID: 2719646
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 2719964
Lung fibroblast, Pubmed ID: 12761501
Lymphocyte, Pubmed ID: 1988031
Mammary cancer, Pubmed ID: 19497882
Melanoma, Pubmed ID: 1386213
Placenta, Pubmed ID: 3065332, 3611046, 10369126, 18824694
Placenta, and Promyelocytic leukemia, Pubmed ID: 2910856
Placenta, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Spleen, Pubmed ID: 2383549
Thalamus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-12-19
Question
Is there a BSA free version of anti-Galectin 1 Monoclonal antibody M00470-1 available?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Galectin 1 Monoclonal antibody M00470-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-12
Question
I have attached the WB image, lot number and protocol we used for lymphocyte using anti-Galectin 1 Monoclonal antibody M00470-1. Please let me know if you require anything else.
R. Evans
Verified customer
Asked: 2019-01-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-23
Question
Is a blocking peptide available for product anti-Galectin 1 Monoclonal antibody (M00470-1)?
Verified Customer
Verified customer
Asked: 2018-06-15
Answer
We do provide the blocking peptide for product anti-Galectin 1 Monoclonal antibody (M00470-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-15
Question
Will anti-Galectin 1 Monoclonal antibody M00470-1 work for IF with lymphocyte?
Verified Customer
Verified customer
Asked: 2018-06-08
Answer
According to the expression profile of lymphocyte, LGALS1 is highly expressed in lymphocyte. So, it is likely that anti-Galectin 1 Monoclonal antibody M00470-1 will work for IF with lymphocyte.
Boster Scientific Support
Answered: 2018-06-08
Question
My question regarding product M00470-1, anti-Galectin 1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-05-02
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00470-1 anti-Galectin 1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-02
Question
I was wanting to use your anti-Galectin 1 Monoclonal antibody for IF for human lymphocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lymphocyte identification?
K. Wu
Verified customer
Asked: 2018-01-11
Answer
You can see on the product datasheet, M00470-1 anti-Galectin 1 Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lymphocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-11
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymphocyte using anti-Galectin 1 Monoclonal antibody M00470-1. Let me know if you need anything else.
D. Carter
Verified customer
Asked: 2017-12-13
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-13
Question
Is this M00470-1 anti-Galectin 1 Monoclonal antibody reactive to the isotypes of LGALS1?
W. Johnson
Verified customer
Asked: 2017-09-01
Answer
The immunogen of M00470-1 anti-Galectin 1 Monoclonal antibody is A synthesized peptide derived from human Galectin 1 May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-01
Question
Would M00470-1 anti-Galectin 1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Zhao
Verified customer
Asked: 2017-06-05
Answer
It shows on the product datasheet, M00470-1 anti-Galectin 1 Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-05
Question
I am looking for using your anti-Galectin 1 Monoclonal antibody for positive regulation of erythrocyte aggregation studies. Has this antibody been tested with western blotting on k562 cell lysate? We would like to see some validation images before ordering.
J. Patel
Verified customer
Asked: 2017-03-02
Answer
We appreciate your inquiry. This M00470-1 anti-Galectin 1 Monoclonal antibody is validated on k562 cell lysate. It is guaranteed to work for IP, IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-03-02
Question
We have been able to see staining in rat adipose tissue. What should we do? Is anti-Galectin 1 Monoclonal antibody supposed to stain adipose tissue positively?
R. Brown
Verified customer
Asked: 2016-06-02
Answer
From literature adipose tissue does express LGALS1. From Uniprot.org, LGALS1 is expressed in adipose tissue, lung, placenta promyelocytic leukemia, hepatoma, placenta, lymphocyte, lung fibroblast, thalamus, placenta skin, brain, platelet, brain, cajal-retzius cell fetal brain cortex, melanoma, spleen, mammary cancer, liver, among other tissues. Regarding which tissues have LGALS1 expression, here are a few articles citing expression in various tissues:
Hepatoma, Pubmed ID: 2719646
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 2719964
Lung fibroblast, Pubmed ID: 12761501
Lymphocyte, Pubmed ID: 1988031
Mammary cancer, Pubmed ID: 19497882
Melanoma, Pubmed ID: 1386213
Placenta, Pubmed ID: 3065332, 3611046, 10369126, 18824694
Placenta, and Promyelocytic leukemia, Pubmed ID: 2910856
Placenta, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Spleen, Pubmed ID: 2383549
Thalamus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2016-06-02
Question
We want to test anti-Galectin 1 Monoclonal antibody M00470-1 on human lymphocyte for research purposes, then I may be interested in using anti-Galectin 1 Monoclonal antibody M00470-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Dhar
Verified customer
Asked: 2016-03-24
Answer
The products we sell, including anti-Galectin 1 Monoclonal antibody M00470-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-03-24
Question
We are currently using anti-Galectin 1 Monoclonal antibody M00470-1 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
C. Krishna
Verified customer
Asked: 2015-10-15
Answer
The anti-Galectin 1 Monoclonal antibody (M00470-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-10-15
Question
We ordered your anti-Galectin 1 Monoclonal antibody for IF on placenta skin in a previous experiment. I am using mouse, and We are going to use the antibody for IP next. I would like examining placenta skin as well as spleen in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
C. Rodriguez
Verified customer
Asked: 2014-07-22
Answer
I have checked the website and datasheets of our anti-Galectin 1 Monoclonal antibody and it seems that M00470-1 has been validated on mouse in both IF and IP. Thus M00470-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-07-22
