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Product Info Summary
| SKU: | A02361-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-GABA A Receptor gamma 2/GABRG2 Antibody Picoband®
View all GABA-A R gamma 2 Antibodies
SKU/Catalog Number
A02361-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GABA A Receptor gamma 2/GABRG2 Antibody Picoband® catalog # A02361-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GABA A Receptor gamma 2/GABRG2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02361-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GABA A Receptor gamma 2/GABRG2 recombinant protein (Position: Q40-R446).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A02361-1 is reactive to GABRG2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
53054 MW
Background of GABA-A R gamma 2
Gamma-aminobutyric acid receptor subunit gamma-2 is a protein that in humans is encoded by the GABRG2 gene. This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammlian brain, where it acts at GABA-A receptors, which are ligand-gated chloride channels. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene have been associated with epilepsy and febrile seizures. Multiple transcript variants encoding different isoforms have been identified for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02361-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: rat brain tissue, rat brain tissue
IHC: mouse brain tissue
FCM: THP-1 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GABA A Receptor gamma 2/GABRG2 using anti-GABA A Receptor gamma 2/GABRG2 antibody (A02361-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABA A Receptor gamma 2/GABRG2 antigen affinity purified polyclonal antibody (Catalog # A02361-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABA A Receptor gamma 2/GABRG2 at approximately 50KD. The expected band size for GABA A Receptor gamma 2/GABRG2 is at 50KD.
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Figure 2. IHC analysis of GABA A Receptor gamma 2/GABRG2 using anti-GABA A Receptor gamma 2/GABRG2 antibody (A02361-1).
GABA A Receptor gamma 2/GABRG2 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GABA A Receptor gamma 2/GABRG2 Antibody (A02361-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. Flow Cytometry analysis of THP-1 cells using anti-GABA A Receptor gamma 2/GABRG2 antibody (A02361-1).
Overlay histogram showing THP-1 cells stained with A02361-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GABA A Receptor gamma 2/GABRG2 Antibody (A02361-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For GABRG2 (Source: Uniprot.org, NCBI)
Gene Name
GABRG2
Full Name
Gamma-aminobutyric acid receptor subunit gamma-2
Weight
53054 MW
Superfamily
ligand-gated ion channel (TC 1.A.9) family
Alternative Names
Neuronal acetylcholine receptor subunit alpha-5;CHRNA5;NACHRA5; GABRG2 CAE2, DEE74, ECA2, EIEE74, FEB8, GEFSP3 gamma-aminobutyric acid type A receptor subunit gamma2 gamma-aminobutyric acid receptor subunit gamma-2|GABA(A) receptor subunit gamma-2|GABA(A) receptor, gamma 2|gamma-aminobutyric acid (GABA) A receptor, gamma 2|gamma-aminobutyric acid type A receptor gamma2 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GABRG2, check out the GABRG2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GABRG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GABA A Receptor gamma 2/GABRG2 Antibody Picoband® (A02361-1)
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